The cell cycle inhibitor
p16(INK4A) is frequently inactivated in acute lymphoblastic
T-cell leukemia (
T-ALL). We analyzed mechanisms and consequences of
p16(INK4A) reconstitution in
T-ALL cells lacking this
tumor suppressor. CCRF-CEM cells with
tetracycline-regulated
p16(INK4A) expression underwent stable G1-phase cell cycle arrest for 72 h followed by massive apoptosis.
p16(INK4A) expression caused pRB hypophosphorylation and repression of certain E2F target genes. Interestingly,
cyclin E and c-Myc were not affected, suggesting pRB/E2F-independent expression of these E2F targets.
Cyclin E/CDK2, however, was inactive due to stabilization and redistribution of p27(Kip1) from CDK4/CDK6 to CDK2. Analyses of c-Myc target genes suggested that c-Myc was transcriptionally inactive, which correlated with hypophosphorylation of the c-Myc inhibitor p107. Thus,
p16(INK4A), although unable to repress the expression of deregulated
cyclin E and c-Myc, functionally inactivated these potential oncogenes. p16(INK4A)-arrested cells showed morphologic changes, induction of T-cell-specific surface markers and repression of
telomerase activity, suggesting differentiation. Moreover,
p16(INK4A) reconstitution was associated with increased cellular volume, normal
protein synthesis rates and elevated
ATP levels. Taken together,
p16(INK4A) reconstitution in p16(INK4A)-deficient
T-ALL cells induced cell cycle arrest in the presence of
cyclin E and c-Myc expression, uncoupled growth from cell cycle progression and caused a sequential process of growth, differentiation and apoptosis.