Abstract |
The objective of this study was to evaluate the influence of irrigants on the antimicrobial potential of calcium hydroxide paste in dogs' teeth with apical periodontitis. Forty-eight premolar teeth of adult mongrel dogs had their root canals opened to the oral environment for 6 months. The root canals were prepared and treated with different irrigating solutions and intracanal medicaments, according to the following groups: 1) 2.5% sodium hypochlorite (NaOCl) + calcium hydroxide paste (CHP); 2) 2% chlorhexidine (CHX) + CHP; 3) vinegar + CHP; 4) vinegar + vinegar. In group 4, both the irrigating solution and intracanal medicament were vinegar, which was renewed every 7 days. Each sample was collected by using two paper points maintained in position for 1 min, and individually transported and immersed in 7 mL of Letheen broth (LB), followed by incubation at 37 degrees C for 48 h. Microbial growth was analyzed by two methods: turbidity of the culture medium and subculture on a specific nutrient broth (brain heart infusion). After 21 days, all experimental groups had microbial growth, however, in different percentages: group 1 - 30%; group 2 - 30%; group 3 - 40%; group 4 - 60%. All materials tested had antimicrobial potential; however, the influence of calcium hydroxide paste on the control of microorganisms must be remembered.
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Authors | Carlos Estrela, Roberto Holland, Pedro Felício Estrada Bernabé, Valdir de Souza, Cyntia R A Estrela |
Journal | Brazilian dental journal
(Braz Dent J)
Vol. 15
Issue 3
Pg. 181-5
( 2004)
ISSN: 1806-4760 [Electronic] Brazil |
PMID | 15798819
(Publication Type: Journal Article)
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Chemical References |
- Anti-Infective Agents, Local
- Root Canal Irrigants
- Sodium Hypochlorite
- Calcium Hydroxide
- Acetic Acid
- Chlorhexidine
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Topics |
- Acetic Acid
(therapeutic use)
- Animals
- Anti-Infective Agents, Local
(therapeutic use)
- Bacteria
(drug effects, growth & development)
- Calcium Hydroxide
(therapeutic use)
- Chlorhexidine
(therapeutic use)
- Dental Pulp Cavity
(drug effects, microbiology)
- Dogs
- Periapical Periodontitis
(microbiology, therapy)
- Root Canal Irrigants
(therapeutic use)
- Root Canal Preparation
(instrumentation, methods)
- Sodium Hypochlorite
(therapeutic use)
- Wound Healing
(drug effects)
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