Increased expression of osteopontin gene in atypical teratoid/rhabdoid tumor of the central nervous system.

Abstract

The atypical teratoid/rhabdoid tumor, primary to the central nervous system, is a highly malignant and aggressive neoplasm of infancy and childhood. Although having distinct biological features and clinical outcomes, it is frequently misdiagnosed as primitive neuroectodermal tumor/medulloblastoma. To further distinguish the underlying pathogenesis and to identify biological markers for clinical use, an atypical teratoid/rhabdoid tumor-derived cell line was established and its gene expression pattern analyzed in comparison to the human astrocyte SVG12 cell line and the human DAOY medulloblastoma cell line using a complementary DNA microarray method. The osteopontin gene was found specifically upregulated in atypical teratoid/rhabdoid tumor cells. This specificity was confirmed by immunohistochemistry in pathological sections of tissues from atypical teratoid/rhabdoid tumor patients. Even though the role of osteopontin in the cytopathogenesis of atypical teratoid/rhabdoid tumor still needs to be determined, our data support that overexpressed osteopontin is a potential diagnostic marker for atypical teratoid/rhabdoid tumor.

• Authors: Chung-Lan Kao, Shih-Hwa Chiou, Yann-Jang Chen, Sher Singh, Han-Tso Lin, Ren-Shyan Liu, Chih-Wen Lo, Chi-Chang Yang, Chin-Wen Chi, Chen-hsen Lee, Tai-Tong Wong
• Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (Mod Pathol) Vol. 18 Issue 6 Pg. 769-78 (Jun 2005) ISSN: 0893-3952 [Print] United States
• PMID: 15776015 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• SPP1 protein, human
• Sialoglycoproteins
• Osteopontin

Topics

• Cell Line
• Cell Line, Tumor
• Central Nervous System Neoplasms (genetics, metabolism, pathology)
• Cluster Analysis
• Gene Expression Profiling
• Gene Expression Regulation, Neoplastic (genetics)
• Humans
• Immunohistochemistry
• Medulloblastoma (genetics, metabolism, pathology)
• Nucleic Acid Hybridization (methods)
• Oligonucleotide Array Sequence Analysis
• Osteopontin
• Reverse Transcriptase Polymerase Chain Reaction
• Rhabdoid Tumor (genetics, metabolism, pathology)
• Sialoglycoproteins (analysis, genetics)
• Teratoma (genetics, metabolism, pathology)