Abstract |
Our recent data demonstrate that activation of pmKATP channels polarizes the membrane of cardiomyocytes and reduces Na+/Ca2+ exchange-mediated Ca2+ overload. However, it is important that these findings be extended into contractile models of hypoxia/reoxygenation injury to further test the notion that pmKATP channel activation affords protection against contractile dysfunction and calcium overload. Single rat heart right ventricular myocytes were enzymatically isolated, and cell contractility and Ca2+ transients in field-stimulated myocytes were measured in a cellular model of metabolic inhibition and reoxygenation. Activation of pmKATP with P-1075 (5 microM) or inhibition of the Na+/Ca2+ exchanger with KB-R7943 (5 microM)reduced reoxygenation-induced diastolic Ca2+ overload and improved the rate and magnitude of posthypoxic contractile recovery during the first few minutes of reoxygenation. Moreover,diastolic Ca2+ overload and posthypoxic contractile dysfunction were aggravated in ventricular myocytes either subjected to specific blockade of pmKATP with HMR1098 (20 microM) or expressing the dominant-negative pmKATP construct Kir6.2(AAA) in the presence of P-1075. Our results suggest that a common mechanism, involving resting membrane potential-modulated increases in diastolic [Ca2+]i, is responsible for the development of contractile dysfunction during reoxygenation following metabolic inhibition. This novel and highly plausible cellular mechanism for pmKATP-mediated cardioprotection may have direct clinical relevance as evidenced by the following findings: a hypokalemic polarizing cardioplegia solution supplemented with the pmKATP opener P-1075 improved Ca2+ homeostasis and recovery of function compared with hyperkalemic depolarizing St. Thomas' cardioplegia following contractile arrest in single ventricular myocytes and working rat hearts. We therefore propose that activation of pmKATP channels improves posthypoxic cardiac function via reductions in abnormal diastolic Ca2+ homeostasis mediated by reverse-mode Na+/Ca2+ exchange.
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Authors | István Baczkó, Lynn Jones, Claire F McGuigan, Jocelyn E Manning Fox, Manoj Gandhi, Wayne R Giles, Alexander S Clanachan, Peter E Light |
Journal | FASEB journal : official publication of the Federation of American Societies for Experimental Biology
(FASEB J)
Vol. 19
Issue 8
Pg. 980-2
(Jun 2005)
ISSN: 1530-6860 [Electronic] United States |
PMID | 15774423
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cardioplegic Solutions
- Kir6.2 channel
- Potassium Channel Blockers
- Potassium Channels
- Potassium Channels, Inwardly Rectifying
- Recombinant Fusion Proteins
- Sodium-Calcium Exchanger
- Green Fluorescent Proteins
- Calcium
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Topics |
- Animals
- Calcium
(analysis)
- Cardioplegic Solutions
- Cell Membrane
(chemistry, physiology)
- Diastole
- Green Fluorescent Proteins
(genetics)
- Heart Ventricles
- Homeostasis
- Hypoxia
- Male
- Membrane Potentials
- Mutagenesis, Site-Directed
- Myocardial Contraction
- Myocardial Reperfusion Injury
- Myocytes, Cardiac
(physiology, ultrastructure)
- Potassium Channel Blockers
(pharmacology)
- Potassium Channels
(physiology)
- Potassium Channels, Inwardly Rectifying
(genetics, physiology)
- Rats
- Rats, Sprague-Dawley
- Recombinant Fusion Proteins
- Sodium-Calcium Exchanger
(antagonists & inhibitors, physiology)
- Transfection
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