Application of synthetic 8-kD and recombinant GP50 antigens in the diagnosis of neurocysticercosis by enzyme-linked immunosorbent assay.

Abstract

The gold standard serodiagnostic assay for cysticercosis and neurocysticercosis, diseases caused by the metacestode of Taenia solium, uses lentil lectin-purified glycoprotein (LLGP) in a Western blot assay. We tested two antigens derived from LLGP, synthetic TS18var1 (sTS18var1) and recombinant GP50 antigen (rGP50), in an enzyme-linked immunosorbent assay (ELISA) using serum and cerebrospinal fluid (CSF) samples. The sensitivity for serum and CSF was 94.7% and 100% for rGP50 and 90.4% and 90.2% for sTS18var1, respectively. The specificity for serum and CSF samples was 93.8% and 100% for rGP50 and 90.3% and 98.0% for sTS18var1, respectively. The use of these antigens individually or combined as a diagnostic antigen cocktail eliminates the need for purification of antigens from parasite material and offers the advantage of using a simple and quantitative ELISA format.

• Authors: Edneia C Bueno, Christina M Scheel, Adelaide J Vaz, Luis R Machado, José A Livramento, Osvaldo M Takayanagui, Victor C W Tsang, Kathy Hancock
• Journal: The American journal of tropical medicine and hygiene (Am J Trop Med Hyg) Vol. 72 Issue 3 Pg. 278-83 (Mar 2005) ISSN: 0002-9637 [Print] United States
• PMID: 15772321 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies
• Nerve Tissue Proteins
• Recombinant Proteins
• brain synaptic membrane glycoprotein gp 50

Topics

• Animals
• Antibodies (blood)
• Enzyme-Linked Immunosorbent Assay (methods)
• Humans
• Nerve Tissue Proteins (blood, genetics)
• Neurocysticercosis (blood, diagnosis, immunology)
• Recombinant Proteins (blood)
• Reference Values
• Sensitivity and Specificity
• Serologic Tests
• Taenia (immunology)
• Taeniasis (blood, diagnosis, immunology)