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Efficacy of DNA vaccination against western equine encephalitis virus infection.

Abstract
The efficacy of a DNA vaccine against western equine encephalitis (WEE) infection in mice was evaluated. The 26S structural region was expressed, in vitro from an internal T7 promoter using a rabbit reticulysate transcription/translation system; and from a CMV promoter after transfection into Vero cell monolayers. The proteins synthesized were reactive with anti-WEE virus (WEEV) antibodies, both in western blot analysis and histochemical staining, respectively. When the DNA vaccine plasmid, pVHX-6, was administered intraepidermally to mice, followed by challenge in a lethal mouse model, the level of protection obtained ranged from 50 to 100% amongst three strains of WEEV. Preliminary results suggest the protective immunity provided by the DNA vaccine appears to be a cell-mediated immune response, as elevated cytotoxic T lymphocyte activity was detected against the E2 protein in a T-cell proliferation assay. The efficacy results suggest a DNA vaccine may be a promising approach against WEE infection.
AuthorsLes P Nagata, Wei-Gang Hu, Saad A Masri, George A Rayner, Fay L Schmaltz, Dipankar Das, Josh Wu, Melissa C Long, Christine Chan, David Proll, Scott Jager, Lellean Jebailey, Mavanur R Suresh, Jonathan P Wong
JournalVaccine (Vaccine) Vol. 23 Issue 17-18 Pg. 2280-3 (Mar 18 2005) ISSN: 0264-410X [Print] Netherlands
PMID15755611 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antigens, Viral
  • Vaccines, DNA
  • Viral Structural Proteins
  • Viral Vaccines
Topics
  • Animals
  • Antigens, Viral (genetics)
  • Chlorocebus aethiops
  • Encephalitis Virus, Western Equine (genetics, immunology)
  • Encephalomyelitis, Equine (immunology, prevention & control)
  • Genetic Vectors
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred BALB C
  • Plasmids (genetics)
  • Rabbits
  • T-Lymphocytes, Cytotoxic (immunology)
  • Vaccines, DNA (genetics, pharmacology)
  • Vero Cells
  • Viral Structural Proteins (genetics, immunology)
  • Viral Vaccines (genetics, pharmacology)

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