Abstract |
The efficacy of a DNA vaccine against western equine encephalitis (WEE) infection in mice was evaluated. The 26S structural region was expressed, in vitro from an internal T7 promoter using a rabbit reticulysate transcription/translation system; and from a CMV promoter after transfection into Vero cell monolayers. The proteins synthesized were reactive with anti-WEE virus (WEEV) antibodies, both in western blot analysis and histochemical staining, respectively. When the DNA vaccine plasmid, pVHX-6, was administered intraepidermally to mice, followed by challenge in a lethal mouse model, the level of protection obtained ranged from 50 to 100% amongst three strains of WEEV. Preliminary results suggest the protective immunity provided by the DNA vaccine appears to be a cell-mediated immune response, as elevated cytotoxic T lymphocyte activity was detected against the E2 protein in a T-cell proliferation assay. The efficacy results suggest a DNA vaccine may be a promising approach against WEE infection.
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Authors | Les P Nagata, Wei-Gang Hu, Saad A Masri, George A Rayner, Fay L Schmaltz, Dipankar Das, Josh Wu, Melissa C Long, Christine Chan, David Proll, Scott Jager, Lellean Jebailey, Mavanur R Suresh, Jonathan P Wong |
Journal | Vaccine
(Vaccine)
Vol. 23
Issue 17-18
Pg. 2280-3
(Mar 18 2005)
ISSN: 0264-410X [Print] Netherlands |
PMID | 15755611
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Viral
- Vaccines, DNA
- Viral Structural Proteins
- Viral Vaccines
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Topics |
- Animals
- Antigens, Viral
(genetics)
- Chlorocebus aethiops
- Encephalitis Virus, Western Equine
(genetics, immunology)
- Encephalomyelitis, Equine
(immunology, prevention & control)
- Genetic Vectors
- Lymphocyte Activation
- Mice
- Mice, Inbred BALB C
- Plasmids
(genetics)
- Rabbits
- T-Lymphocytes, Cytotoxic
(immunology)
- Vaccines, DNA
(genetics, pharmacology)
- Vero Cells
- Viral Structural Proteins
(genetics, immunology)
- Viral Vaccines
(genetics, pharmacology)
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