Abstract |
DNA vaccine against M-CSFR(J6-1) ( macrophage colony-stimulating factor receptor cloned from the J6-1 leukemic cell line) has shown both protective and therapeutic effects. In this study, to explore the adjuvant effects of LL-37 to M-CSFR(J6-1) DNA vaccines, we constructed genetically fused vaccines encoding M-CSFR(J6-1) and LL-37(pF). After immunizing BALB/c mice, specific humoral and cellular immune responses were detected. Compared with pR (encoding the extracellular region of M-CSFR(J6-1)), pF was more effective in inducing humoral and cytotoxic immune response, prolonging survival of mice challenged with SP2/0-CSFR(J6-1) tumor cells, and inducing IFN-gamma and IL-4 release by splenocytes. In this study, we also constructed pLL37 (encoding the mature LL-37) and coadministrated pLL37 and pR to see whether the genetic fusion was necessary. We found that compared with pR alone, pLL37+pR could not prolong survival of mice challenged with SP2/0-CSFR(J6-1) tumor cells. Our results suggest that when genetically fused with M-CSFR(J6-1), LL-37 could enhance adaptive immune response against M-CSFR(J6-1) in a murine model challenged with tumor cells bearing M-CSFR(J6-1).
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Authors | Li-Li An, Ying-Hua Yang, Xiao-Tong Ma, Yong-Min Lin, Ge Li, Yu-Hua Song, Ke-Fu Wu |
Journal | Leukemia research
(Leuk Res)
Vol. 29
Issue 5
Pg. 535-43
(May 2005)
ISSN: 0145-2126 [Print] England |
PMID | 15755506
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adjuvants, Immunologic
- Antimicrobial Cationic Peptides
- Cytokines
- Lipopolysaccharides
- Recombinant Fusion Proteins
- Vaccines, DNA
- Receptor, Macrophage Colony-Stimulating Factor
- Cathelicidins
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Topics |
- Adaptation, Physiological
- Adjuvants, Immunologic
- Animals
- Antimicrobial Cationic Peptides
(genetics)
- COS Cells
- Chemotaxis
- Chlorocebus aethiops
- Cytokines
(metabolism)
- Female
- Immune System
(physiology)
- Immunization
- Leukemia
(immunology, metabolism, therapy)
- Lipopolysaccharides
- Mice
- Mice, Inbred BALB C
- Plasmids
- Receptor, Macrophage Colony-Stimulating Factor
(genetics)
- Recombinant Fusion Proteins
(genetics)
- Survival Rate
- T-Lymphocytes, Cytotoxic
(immunology)
- Vaccines, DNA
(therapeutic use)
- Cathelicidins
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