HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

High cytotoxic sensitivity of the human small cell lung doxorubicin-resistant carcinoma (GLC4/ADR) cell line to prodigiosin through apoptosis activation.

Abstract
In the present study, we describe the cytotoxicity of the new drug prodigiosin (PG) in two small cell lung carcinoma (SCLC) cell lines, GLC4 and its derived doxorubicin-resistant GLC4/ADR cell line, which overexpresses multidrug-related protein 1 (MRP-1). We observed through Western blot that PG mediated cytochrome c release, caspase cascade activation and PARP cleavage, thereby leading to apoptosis in a dose-response manner. MRP-1 expression increased after PG treatment, although that does not lead to protein accumulation. The MTT assay showed no difference in sensitivity to PG between the two cell lines. Our results support PG as a potential drug for the treatment of lung cancer as it overcomes the multidrug resistance phenotype produced by MRP-1 overexpression.
AuthorsEsther Llagostera, Vanessa Soto-Cerrato, Ricky Joshi, Beatriz Montaner, Pepita Gimenez-Bonafé, Ricardo Pérez-Tomás
JournalAnti-cancer drugs (Anticancer Drugs) Vol. 16 Issue 4 Pg. 393-9 (Apr 2005) ISSN: 0959-4973 [Print] England
PMID15746575 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Anti-Bacterial Agents
  • Antibiotics, Antineoplastic
  • Doxorubicin
  • Cytochromes c
  • Poly(ADP-ribose) Polymerases
  • Caspases
  • Prodigiosin
Topics
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 (metabolism)
  • Anti-Bacterial Agents (therapeutic use)
  • Antibiotics, Antineoplastic (therapeutic use)
  • Apoptosis (drug effects)
  • Carcinoma, Small Cell (drug therapy, pathology)
  • Caspases (metabolism)
  • Cytochromes c (metabolism)
  • Doxorubicin (therapeutic use)
  • Drug Resistance, Neoplasm
  • Enzyme Activation (drug effects)
  • Humans
  • Lung Neoplasms (drug therapy, pathology)
  • Poly(ADP-ribose) Polymerases (metabolism)
  • Prodigiosin (therapeutic use)
  • Tumor Cells, Cultured

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: