Efficacy of vaccination of calves against hemorrhagic septicemia with a live aroA derivative of Pasteurella multocida B:2 by two different routes of administration.

Abstract

Two groups of four calves each were immunized either intramuscularly (i.m. vaccinated) or intranasally (i.n. vaccinated) at 2 and 6 weeks of age with ca. 10(9) CFU of a derivative of P. multocida serotype B:2 strain 85020 containing a deletion in the aroA gene (strain JRMT12). Both groups of calves and three unvaccinated control calves were challenged subcutaneously at 8 weeks of age with ca. 10(7) CFU of the wild-type 85020 strain. The first and second vaccinations caused a significant pyrexia and increase in the mean demeanor score (P <0.05) in i.m. but not i.n. vaccinated calves. Serum agglutinating activity against whole cells of P. multocida strain 85020 and immunoglobulin G antibody concentrations increased after the second vaccination in i.m. but not in i.n. vaccinated animals, and this difference was statistically significant (P <0.05). Concentrations of serum amyloid A (SAA) increased significantly 3 h after both the primary (P <0.05) and booster (P <0.001) i.m. vaccinations, but not in i.n. vaccinated calves. All four i.m. vaccinated calves were solidly immune to challenge with wild-type P. multocida B:2. However, the mean rectal temperatures, demeanor scores, and serum SAA concentrations of i.n. vaccinated and control calves increased significantly (P <0.01). Three i.n. vaccinated and two control calves were killed for humane reasons within 14 h postchallenge, and postmortem examination revealed pathological lesions consistent with hemorrhagic septicemia. These data showed that the aroA mutant strain, given i.m. as two doses 4 weeks apart, acted as an effective live-attenuated vaccine strain to protect calves against challenge with the virulent parent strain.

• Authors: J Christopher Hodgson, Anna Finucane, Mark P Dagleish, Saeed Ataei, Roger Parton, John G Coote
• Journal: Infection and immunity (Infect Immun) Vol. 73 Issue 3 Pg. 1475-81 (Mar 2005) ISSN: 0019-9567 [Print] United States
• PMID: 15731045 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Bacterial
• Bacterial Vaccines
• Serum Amyloid A Protein
• Vaccines, Attenuated
• Alkyl and Aryl Transferases
• 3-Phosphoshikimate 1-Carboxyvinyltransferase

Topics

• 3-Phosphoshikimate 1-Carboxyvinyltransferase
• Administration, Intranasal
• Alkyl and Aryl Transferases (genetics, immunology)
• Animals
• Antibodies, Bacterial (blood)
• Bacterial Vaccines (administration & dosage, immunology)
• Cattle
• Cattle Diseases (mortality, prevention & control)
• Hemorrhagic Septicemia (mortality, prevention & control, veterinary)
• Immunization Schedule
• Injections, Intramuscular
• Pasteurella Infections (mortality, prevention & control, veterinary)
• Pasteurella multocida (classification, genetics, immunology, pathogenicity)
• Serotyping
• Serum Amyloid A Protein (analysis)
• Treatment Outcome
• Vaccination (veterinary)
• Vaccines, Attenuated (administration & dosage, immunology)