Involvement of NO in the convulsive behavior and oxidative damage induced by the intrastriatal injection of methylmalonate.

Abstract

Acute intrastriatal administration of methylmalonic acid (MMA) induces convulsions through NMDA receptor-mediated mechanisms and increases production of end products of oxidative damage. Although it has been demonstrated that nitric oxide (NO) production increases with NMDA receptor stimulation and contributes to the oxidative damage observed in several neurodegenerative disorders, the role of NO in MMA-induced convulsions has not been investigated to date. In the present study we investigated the effects of the intrastriatal injection of N(omega)-nitro-L-arginine methyl ester (L-NAME: 10(-4) to 10(0) nmol/0.5 microl) on the convulsions and striatal protein carbonylation induced by the intrastriatal injection of MMA (4.5 micromol/1.5 microl). l-NAME (10(-3) to 10(-1)nmol) protected against MMA-induced convulsions and protein carbonylation ex vivo. These results suggest the involvement of NO in the convulsive behavior and protein carbonylation elicited by MMA.

• Authors: Luiz Fernando Freire Royes, Michele Rechia Fighera, Ana Flávia Furian, Mauro Schneider Oliveira, Natália Gindri Fiorenza, Jociane de Carvalho Myskiw, Roberto Frussa-Filho, Carlos Fernando Mello
• Journal: Neuroscience letters (Neurosci Lett) Vol. 376 Issue 2 Pg. 116-20 (Mar 11 2005) ISSN: 0304-3940 [Print] Ireland
• PMID: 15698932 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Enzyme Inhibitors
• Nitric Oxide
• Methylmalonic Acid
• NG-Nitroarginine Methyl Ester

Topics

• Animals
• Enzyme Inhibitors (pharmacology)
• Injections, Intraventricular
• Male
• Methylmalonic Acid (administration & dosage)
• NG-Nitroarginine Methyl Ester (pharmacology)
• Nitric Oxide (metabolism)
• Oxidative Stress (physiology)
• Rats
• Rats, Wistar
• Seizures (chemically induced)