Abstract | OBJECTIVE: METHODS: A panel of 10 anti-dsDNA and/or anti- alpha-actinin antibodies was generated by Epstein-Barr virus transformation of lymphocytes from patients with SLE and was extensively characterized. Antibody binding was studied by enzyme-linked immunosorbent assay and Western blotting. Antibody potential for pathogenicity was assessed by measuring binding to isolated glomeruli and mesangial cells and by evaluation of histologic features of the kidney following injection in vivo. RESULTS: All anti-dsDNA antibodies isolated also bound alpha-actinin. Cross-reactive antibodies bound to mesangial cells and to isolated glomeruli ex vivo. Binding to glomeruli was not inhibited by DNase treatment, but could be abrogated by alpha-actinin. Furthermore, histopathologic abnormalities seen in mice injected intraperitoneally with a cross-reactive cell line included fusion of podocyte foot processes and subepithelial and subendothelial deposition. CONCLUSION:
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Authors | Zeguo Zhao, Elena Weinstein, Marina Tuzova, Anne Davidson, Peter Mundel, Paola Marambio, Chaim Putterman |
Journal | Arthritis and rheumatism
(Arthritis Rheum)
Vol. 52
Issue 2
Pg. 522-30
(Feb 2005)
ISSN: 0004-3591 [Print] United States |
PMID | 15693007
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antibodies, Antinuclear
- Autoantibodies
- Immunoglobulin G
- Actinin
- DNA
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Topics |
- Actinin
(immunology)
- Adult
- Animals
- Antibodies, Antinuclear
(immunology)
- Autoantibodies
(analysis)
- Blotting, Western
- Cross Reactions
- DNA
(immunology)
- Enzyme-Linked Immunosorbent Assay
- Female
- Flow Cytometry
- Glomerular Mesangium
(immunology)
- Humans
- Immunoglobulin G
(analysis)
- Immunohistochemistry
- Kidney
(immunology)
- Kidney Glomerulus
(immunology)
- Lupus Erythematosus, Systemic
(immunology)
- Lupus Nephritis
(immunology)
- Male
- Mice
- Mice, Inbred BALB C
- Middle Aged
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