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Cleavage of integrin by mu-calpain during hypoxia in human endometrial cells.

AbstractPROBLEM:
The distribution and activation of mu-calpain and possible cleavage of integrin in human endometrial cells under hypoxic condition were investigated.
METHOD OF STUDY:
Human endometrial epithelial and stromal cells were subjected to hypoxia, and subsequently used for immunostaining and western blot analysis.
RESULTS:
The proform of mu-calpain was detected in the cytoplasm of normal cells, and displayed a substantial decrease after hypoxia. Conversely, the active form of mu-calpain was not detected in normal cells, but was abundant after hypoxia. The cytoplasmic domain of integrin beta3 was also detected in the cytoplasm of endometrial cells. Western blot analysis confirmed that both the proform of mu-calpain and the integrin beta3 cytoplasmic domain decreased during hypoxia.
CONCLUSIONS:
Mu-calpain is activated in human endometrial cells during hypoxia and that subsequent cleavage of the integrin beta3 cytoplasmic domain may give some adverse effects to the function of human endometrium.
AuthorsKazufumi Aoyama, Yasuhiko Ozaki, Tamao Nakanishi, Mayumi S Ogasawara, Katsuo Ikuta, Koji Aoki, Klas Blomgren, Kaoru Suzumori
JournalAmerican journal of reproductive immunology (New York, N.Y. : 1989) (Am J Reprod Immunol) Vol. 52 Issue 6 Pg. 362-9 (Dec 2004) ISSN: 1046-7408 [Print] Denmark
PMID15663601 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Copyright(c) Blackwell Munksgaard, 2004
Chemical References
  • Integrin beta3
  • Calpain
  • mu-calpain
Topics
  • Blotting, Western
  • Calpain (metabolism)
  • Cell Hypoxia (physiology)
  • Cell Survival
  • Cells, Cultured
  • Electrophoresis, Polyacrylamide Gel
  • Endometrium (cytology, metabolism)
  • Female
  • Humans
  • Immunohistochemistry
  • Integrin beta3 (metabolism)
  • Microscopy, Confocal

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