Abstract | BACKGROUND: Biochemical analysis of expiratory breath condensate is an emerging non-invasive technique for assessment of airway inflammation. OBJECTIVE: We wondered whether application of expiratory breath condensate could facilitate diagnosis of aspirin-intolerant asthma and reproduce eicosanoids mediators' abnormalities described in this disease. METHODS: We measured prostaglandins (PGs) E(2), F(2 alpha), 9 alpha 11 beta F(2) and iso-F(2) by gas-chromatography/mass-spectrometry and cysteinyl leukotrienes (cys-LTs) by radioimmunoassay in breath condensates of asthmatic patients undergoing oral aspirin challenge. Fourteen patients with aspirin-induced asthma and 20 aspirin-tolerating asthmatics, most of them on chronic inhaled corticotherapy, were studied and compared with 10 healthy subjects. Additionally, plasma 9 alpha 11 beta PGF(2), the metabolite of PGD(2) and urinary leukotriene (LT) E(4) were measured before and following the challenge. RESULTS: At baseline, PG did not differ between the groups, except for lower 9 alpha 11 beta PGF(2) in aspirin-intolerant asthma. Their concentrations were not changed by the challenge. Breath condensate cys-LTs were similar in the groups studied at base, and after aspirin challenge increased only in aspirin-intolerant patients. Elevated baseline urinary LTE(4) and its further increase following aspirin challenge was highly diagnostic for aspirin-intolerant asthma. The discriminatory value of cys-LTs increase in breath condensates was lower (72.8%) than either basal (99%) or post-challenge increase (94%) of urinary LTE(4). CONCLUSIONS: In asthmatic patients on chronic corticotherapy measurement of urinary LTE(4) excretion rather than cys-LTs in breath condensate is of greater value for diagnosis of aspirin hypersensitivity.
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Authors | M Sanak, B Kiełbasa, G Bochenek, A Szczeklik |
Journal | Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
(Clin Exp Allergy)
Vol. 34
Issue 12
Pg. 1899-904
(Dec 2004)
ISSN: 0954-7894 [Print] England |
PMID | 15663565
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Anti-Inflammatory Agents, Non-Steroidal
- Biomarkers
- Eicosanoids
- Leukotriene E4
- Dinoprost
- Dinoprostone
- Aspirin
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Topics |
- Adult
- Anti-Inflammatory Agents, Non-Steroidal
(adverse effects)
- Area Under Curve
- Aspirin
(adverse effects)
- Asthma
(chemically induced, diagnosis)
- Biomarkers
(analysis, blood, urine)
- Breath Tests
- Case-Control Studies
- Dinoprost
(analysis, blood)
- Dinoprostone
(analysis)
- Eicosanoids
(analysis)
- Female
- Gas Chromatography-Mass Spectrometry
- Humans
- Leukotriene E4
(urine)
- Lung
(drug effects, metabolism)
- Male
- Middle Aged
- ROC Curve
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