Abstract |
Borna disease virus (BDV) can persistently infect the central nervous system and induce CD8+ T-cell-mediated neurological disease in MRL mice. To determine whether specific immune priming would prevent disease, a prime-boost immunization protocol was established in which intramuscular injection of a recombinant parapoxvirus expressing BDV nucleoprotein (BDV-N) was followed by intraperitoneal infection with vaccinia virus expressing BDV-N. Immunized wild-type and perforin-deficient mice remained healthy after intracerebral infection with BDV and contained almost no virus in the brain at 5 weeks post-challenge. Immunization failed to induce resistance against BDV in mice lacking mature CD8+ T cells. Immunization of perforin-deficient mice with a poxvirus vector expressing mutant BDV-N lacking the known CD8+ T-cell epitope did not efficiently block multiplication of BDV in the brain and did not prevent neurological disease, indicating that vaccine-induced immunity to BDV in wild-type and perforin-deficient mice resulted from the action of CD8+ T cells.
|
Authors | Jürgen Hausmann, Karen Baur, Karin R Engelhardt, Timo Fischer, Hanns-Joachim Rziha, Peter Staeheli |
Journal | The Journal of general virology
(J Gen Virol)
Vol. 86
Issue Pt 2
Pg. 399-403
(Feb 2005)
ISSN: 0022-1317 [Print] England |
PMID | 15659759
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Epitopes, T-Lymphocyte
- Membrane Glycoproteins
- Nucleoproteins
- Pore Forming Cytotoxic Proteins
- Vaccines, Synthetic
- Viral Proteins
- Viral Vaccines
- Perforin
|
Topics |
- Animals
- Borna Disease
(immunology, prevention & control, virology)
- Borna disease virus
(genetics, immunology)
- Brain
(virology)
- CD8-Positive T-Lymphocytes
(immunology)
- Disease Models, Animal
- Drug Evaluation, Preclinical
- Epitopes, T-Lymphocyte
(genetics, immunology)
- Genetic Vectors
- Injections, Intramuscular
- Membrane Glycoproteins
(deficiency, genetics)
- Mice
- Mice, Knockout
- Mutation
- Nucleoproteins
(biosynthesis, genetics)
- Parapoxvirus
(genetics)
- Perforin
- Pore Forming Cytotoxic Proteins
- Vaccination
- Vaccines, Synthetic
(administration & dosage)
- Viral Proteins
(biosynthesis, genetics)
- Viral Vaccines
(administration & dosage)
|