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Vaccine-induced protection against Borna disease in wild-type and perforin-deficient mice.

Abstract
Borna disease virus (BDV) can persistently infect the central nervous system and induce CD8+ T-cell-mediated neurological disease in MRL mice. To determine whether specific immune priming would prevent disease, a prime-boost immunization protocol was established in which intramuscular injection of a recombinant parapoxvirus expressing BDV nucleoprotein (BDV-N) was followed by intraperitoneal infection with vaccinia virus expressing BDV-N. Immunized wild-type and perforin-deficient mice remained healthy after intracerebral infection with BDV and contained almost no virus in the brain at 5 weeks post-challenge. Immunization failed to induce resistance against BDV in mice lacking mature CD8+ T cells. Immunization of perforin-deficient mice with a poxvirus vector expressing mutant BDV-N lacking the known CD8+ T-cell epitope did not efficiently block multiplication of BDV in the brain and did not prevent neurological disease, indicating that vaccine-induced immunity to BDV in wild-type and perforin-deficient mice resulted from the action of CD8+ T cells.
AuthorsJürgen Hausmann, Karen Baur, Karin R Engelhardt, Timo Fischer, Hanns-Joachim Rziha, Peter Staeheli
JournalThe Journal of general virology (J Gen Virol) Vol. 86 Issue Pt 2 Pg. 399-403 (Feb 2005) ISSN: 0022-1317 [Print] England
PMID15659759 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Epitopes, T-Lymphocyte
  • Membrane Glycoproteins
  • Nucleoproteins
  • Pore Forming Cytotoxic Proteins
  • Vaccines, Synthetic
  • Viral Proteins
  • Viral Vaccines
  • Perforin
Topics
  • Animals
  • Borna Disease (immunology, prevention & control, virology)
  • Borna disease virus (genetics, immunology)
  • Brain (virology)
  • CD8-Positive T-Lymphocytes (immunology)
  • Disease Models, Animal
  • Drug Evaluation, Preclinical
  • Epitopes, T-Lymphocyte (genetics, immunology)
  • Genetic Vectors
  • Injections, Intramuscular
  • Membrane Glycoproteins (deficiency, genetics)
  • Mice
  • Mice, Knockout
  • Mutation
  • Nucleoproteins (biosynthesis, genetics)
  • Parapoxvirus (genetics)
  • Perforin
  • Pore Forming Cytotoxic Proteins
  • Vaccination
  • Vaccines, Synthetic (administration & dosage)
  • Viral Proteins (biosynthesis, genetics)
  • Viral Vaccines (administration & dosage)

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