Mixed bacterial infection model of sepsis in rabbits and its application to evaluate superantigen-adsorbing device.

Abstract

BACKGROUND:

Superantigens are suspected to be the potent and lethal pathogens of gram-positive sepsis, and a new therapy that targeted to superantigens are required.

METHODS:

A mixed infection model was developed in rabbits by the cecal ligation and puncture associated with the intraperitoneal injection of Staphylococcus aureus, which produces toxic shock syndrome toxin 1 (TSST-1). Animals were also hemoperfused with a superantigen-adsorbing device (SAAD), or a control column.

RESULTS:

The model animals revealed multiple organ failure and died 6-12 h after the injection of S. aureus. The plasma levels of TSST-1, but not of lipopolysaccharide (LPS), significantly (p < 0.01) and inversely correlated with mean arterial pressure (r = -0.63). Plasma TSST-1 level was significantly reduced and shock-onset time was significantly retarded in the SAAD treated group, although the survival time was not significantly affected.

CONCLUSIONS:

The animal model developed could serve as a model for sepsis. It is suggested that there is the potential application of SAAD in treating superantigen-related sepsis.

• Authors: Mayumi Fukuyama, Keishi Miwa, Naoko Shibayama, Shinji Ogura, Takashi Nishiyama, Nobuhiro Maekawa
• Journal: Blood purification (Blood Purif) Vol. 23 Issue 2 Pg. 119-27 ( 2005) ISSN: 0253-5068 [Print] Switzerland
• PMID: 15640604 (Publication Type: Journal Article)

Chemical References

• Bacterial Toxins
• Enterotoxins
• Lipopolysaccharides
• Superantigens
• enterotoxin F, Staphylococcal

Topics

• Adsorption
• Animals
• Bacterial Infections
• Bacterial Toxins (blood)
• Blood Pressure
• Disease Models, Animal
• Enterotoxins (blood)
• Hemoperfusion (methods)
• Lipopolysaccharides (blood)
• Male
• Rabbits
• Sepsis (therapy)
• Staphylococcus aureus
• Superantigens (blood, isolation & purification)
• Survival Rate
• Treatment Outcome