[Protective effects of modified recombinant human acidic fibroblast growth factor on small intestine after ischemia/reperfusion injury in rats].

Abstract

OBJECTIVE:

To explore the protective effect of modified recombinant human acidic fibroblast growth factor (rhaFGF) on small intestinal after ischemia/reperfusion (I/R) injury in rats.

METHODS:

The clamp on the superior mesenteric artery (SMA) was removed after clamping it for 45 minutes to replicate I/R injury of the intestine in the rat. Rats were then divided randomly into sham operation group, normal saline treatment group and rhaFGF treatment group, in which the rats of the normal saline treatment group were injected 0.1 ml of normal saline and that of the rhaFGF group were given 4 microg of rhaFGF immediately after reperfusion. The content of D-lactate in the plasma was determined and the changes in intestinal pathology were observed. At the same time, the rates of apoptosis of intestinal epithelial cells were assessed 2, 6, 12 and 24 hours after I/R, and compared to the sham operation group.

RESULTS:

The plasma content of D-lactate in the saline treatment group at 6 hours after I/R reached (0.34+/-0.09) mg/L and was significantly higher than that in the rhaFGF treatment group((0.23+/-0.07)mg/L, P<0.05). It was shown histologically that the intestinal structures were damaged more seriously in saline treatment group than in rhaFGF group. The apoptosis rates in the saline treatment group and rhaFGF group were elevated significantly, peaking at 12 hours after I/R injury((62.8+/-1.7)% in saline group and (42.5+/-2.6)% in rhaFGF treatment group), then the rate began to fall. There was statistically significant difference between the two groups at 12 hours after I/R injury.

CONCLUSION:

rhaFGF can reduce content of D-lactate in the plasma and rate of apoptosis of epithelial cells in the intestine after I/R injury, thus it seems to afford a protective effect on the small intestine after I/R injury in rats.

• Authors: Tong-zhu Sun, Xiao-bing Fu, Wei Chen, Shu-yun Zheng, Li-xin Weng, Jing-yu Zhao, Jun-you Li, Dan Sun
• Journal: Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue (Zhongguo Wei Zhong Bing Ji Jiu Yi Xue) Vol. 17 Issue 1 Pg. 46-8 (Jan 2005) ISSN: 1003-0603 [Print] China
• PMID: 15636714 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fibroblast Growth Factor 1
• Lactic Acid

Topics

• Animals
• Apoptosis
• Disease Models, Animal
• Epithelial Cells (pathology)
• Fibroblast Growth Factor 1 (pharmacology)
• Humans
• Intestine, Small (blood supply, drug effects, metabolism, pathology)
• Lactic Acid (blood)
• Male
• Random Allocation
• Rats
• Rats, Wistar
• Reperfusion Injury (metabolism, pathology, prevention & control)