Basic evaluation of an immunoradiometric competitive inhibition assay for sialosyl-Tn antigen in sera in women. Assay conditions and normal values.

Abstract	The assay conditions needed for an immunoradiometric competitive inhibition assay of sera in healthy women were studied using the monoclonal antibody TKH2, which is known to recognize specifically sialosyl-alpha 2,6-GalNAc alpha 1-0-serine/threonine (S-Tn) antigen, a mucinous cancer-related antigen. Stable results were obtained with an incubation time of 1.5 hours at room temperature. The intra-assay and inter-assay coefficients of variation were 3.27% and 3.07%, respectively. The mean (+/- standard deviation [SD]) levels of serum S-Tn in 602 healthy women was 21.2 U/ml (+/- 8.4 U/ml). Values showed a normal logarithmic distribution. Although slightly higher levels were seen in postmenopausal compared with premenopausal women, the differences were not significant. The cutoff value of 41 U/ml was determined from data obtained in 602 healthy women; higher levels were observed in only 2%. Serum S-Tn levels were not strongly influenced by Lewis or ABO (H) blood type, smoking, pregnancy, parturition, or phase of menstrual cycle. The use of the S-Tn antigen as a tumor marker for various gynecologic cancers requires study.
Authors	K Ryuko, O Iwanari, S Nakayama, M Kitao, K Karino, J Endo
Journal	Cancer (Cancer) Vol. 69 Issue 9 Pg. 2361-7 (May 01 1992) ISSN: 0008-543X [Print] United States
PMID	1562984 (Publication Type: Journal Article)
Chemical References	ABO Blood-Group System Antibodies, Monoclonal Antigens, Neoplasm Antigens, Tumor-Associated, Carbohydrate Lewis Blood Group Antigens Reagent Kits, Diagnostic sialosyl-Tn antigen
Topics	ABO Blood-Group System (immunology) Adult Aged Antibodies, Monoclonal Antigens, Neoplasm (blood) Antigens, Tumor-Associated, Carbohydrate Binding, Competitive Evaluation Studies as Topic Female Humans Lewis Blood Group Antigens (immunology) Menopause (blood, immunology) Middle Aged Postpartum Period (blood, immunology) Pregnancy (blood, immunology) Radioimmunoassay Reagent Kits, Diagnostic Reference Values

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!