Characterization of a self-assembled monolayer of thiol on a gold surface and the fabrication of a biosensor chip based on surface plasmon resonance for detecting anti-GAD antibody.
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| Abstract |
A biosensor chip utilizing surface plasmon resonance (SPR) was fabricated for detecting anti-glutamic acid decarboxylase (GAD) antibody, which is an indicator of the presence of type I diabetes mellitus. The sensor surfaces were constructed from various thiol mixtures of different molar ratios of 3-mercaptopropionic acid (3-MPA) to 11-mercaptoundecanoic acid (11-MUA). To determine the surface characteristics of the different alkanethiol monolayers, several quantitative and kinetic measurements were carried out. The extent of immobilization of streptavidin (SA) and biotin-GAD (the anti-GAD receptor) and the immune response of anti-GAD antibody against GAD were measured using the SPR biosensor. The terminal functional group of a thiol has different effects on the adsorption and covalent binding of protein depending on the steric hindrance. The protein chip described herein permits simple, rapid detection of anti-GAD antibody.
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| Authors | Jung Wook Lee, Sang Jun Sim, Sung Min Cho, Jeewon Lee |
| Journal | Biosensors & bioelectronics (Biosens Bioelectron) Vol. 20 Issue 7 Pg. 1422-7 (Jan 15 2005) ISSN: 0956-5663 [Print] England |
| PMID | 15590298 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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