Abstract | PURPOSE: To demonstrate the efficacy of repeated, non-invasive optical imaging of reporter gene expression in monitoring the ability of bi-specific recombinant molecules (i) to "transductionally untarget" adenovirus from Coxsackie and Adenovirus Receptor (CAR)-dependent infection of normal tissue and (ii) to "transductionally retarget" infection to specific target cells. PROCEDURES: RESULTS: Systemic injection of sCAR- EGF "coated" adenovirus expressing firefly luciferase from the CMV early promoter, reduces expression of the reporter gene in the liver and facilitates expression of the reporter gene in tumor xenografts expressing high levels of the EGF-receptor. CONCLUSION: Both liver "untargeting" and tumor "retargeting" of adenovirus by recombinant sCAR- EGF can be imaged non-invasively using a luciferase reporter gene.
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Authors | Qianwa Liang, Masato Yamamoto, David T Curiel, Harvey R Herschman |
Journal | Molecular imaging and biology
(Mol Imaging Biol)
2004 Nov-Dec
Vol. 6
Issue 6
Pg. 395-404
ISSN: 1536-1632 [Print] United States |
PMID | 15564150
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Membrane Proteins
- Luciferases, Firefly
- Cyclooxygenase 2
- PTGS2 protein, human
- Prostaglandin-Endoperoxide Synthases
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Topics |
- Adenoviridae
(genetics, isolation & purification, physiology)
- Animals
- Cell Line, Tumor
- Cyclooxygenase 2
- Genes, Reporter
(genetics)
- Genetic Therapy
- Humans
- Injections, Intravenous
- Luciferases, Firefly
(analysis, genetics)
- Lung Neoplasms
(genetics)
- Membrane Proteins
- Mice
- Mice, Nude
- Neoplasm Transplantation
- Neoplasms
(genetics, metabolism, therapy, virology)
- Organ Specificity
- Promoter Regions, Genetic
(genetics)
- Prostaglandin-Endoperoxide Synthases
(analysis, genetics)
- Transcription, Genetic
(genetics)
- Transduction, Genetic
- Transgenes
(genetics)
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