Differentiation factor/leukemia inhibitory factor protection against lethal endotoxemia in mice: synergistic effect with interleukin 1 and tumor necrosis factor.

Abstract

Differentiation factor (D factor), also called leukemia inhibitory factor (LIF), is a glycoprotein that has been increasingly recognized to possess a wide range of physiological activities. We examined the possibility that the administration of D factor may confer beneficial effects and enhance host resistance against lethal endotoxemia. A single intravenous dose of recombinant human D factor completely protected C57/Bl6 mice from the lethal effect of Escherichia coli endotoxin (lipopolysaccharide [LPS]). The protective effects were dose dependent and observed when administered 2-24 h before LPS. Previous work has shown that interleukin 1 (IL-1) and tumor necrosis factor (TNF) also protect against a subsequent LPS challenge in a dose-dependent manner. When human D factor was combined with sub-protective doses of IL-1 beta or TNF-alpha, there was dramatic synergistic protection against a subsequent lethal LPS challenge.

• Authors: H R Alexander, G G Wong, G M Doherty, D J Venzon, D L Fraker, J A Norton
• Journal: The Journal of experimental medicine (J Exp Med) Vol. 175 Issue 4 Pg. 1139-42 (Apr 01 1992) ISSN: 0022-1007 [Print] United States
• PMID: 1552284 (Publication Type: Journal Article)

Chemical References

• Growth Inhibitors
• Interleukin-1
• Interleukin-6
• LIF protein, human
• Leukemia Inhibitory Factor
• Lif protein, mouse
• Lipopolysaccharides
• Lymphokines
• Recombinant Proteins
• Tumor Necrosis Factor-alpha

Topics

• Animals
• Drug Synergism
• Female
• Growth Inhibitors (administration & dosage)
• Interleukin-1 (administration & dosage)
• Interleukin-6
• Leukemia Inhibitory Factor
• Lipopolysaccharides (toxicity)
• Lymphokines (administration & dosage)
• Mice
• Mice, Inbred C57BL
• Recombinant Proteins
• Tumor Necrosis Factor-alpha (administration & dosage)