[Correlation of MUC1 expression to adhesion of breast cancer cell line MDA-MB-231].

Abstract

BACKGROUND & OBJECTIVE:

Recent researches found that an abundant production of mucin protein well correlates with tumor cell metastasis. This study was to investigate inhibitory effect of benzyl-alpha-GalNAc on production of mucin 1 (MUC1), and on adhesion and invasion of breast cancer cell line MDA-MB-231.

METHODS:

MDA-MB-231 cells were incubated with benzyl-alpha-GalNAc, expression of MUC1 was detected by immunohistochemistry, adhesive ability of MDA-MB-231 cells to artificial basement membrane Matrigel was measured by MTT assay. Gelatin zymography was performed to detect the secretion changes of matrix metalloproteinase-2 (MMP-2) and MMP-9.

RESULTS:

Compared with control cells, the tumor cells deglycosylated by benzyl-alpha-GalNAc showed lower expression of MUC1 (P < 0.05), and less adhesion to the Matrigel (P< 0.01), the secretion of MMP-2 and MMP-9 suppressed (P< 0.05).

CONCLUSION:

The expression of MUC1 correlates to adhesion and invasion of MDA-MB-231 cells, benzyl-alpha-GalNAc may weaken adhesion and type IV collagenase-secreting activity of MDA-MB-231 cells by inhibiting MUC1.

• Authors: Li Lu, Hua-Yu Deng, Wei-Ke Fan
• Journal: Ai zheng = Aizheng = Chinese journal of cancer (Ai Zheng) Vol. 23 Issue 11 Pg. 1294-6 (Nov 2004) China
• PMID: 15522176 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benzyl Compounds
• Mucin-1
• benzyl-alpha-N-acetylgalactosamine
• Matrix Metalloproteinase 2
• Matrix Metalloproteinase 9
• Acetylgalactosamine

Topics

• Acetylgalactosamine (analogs & derivatives, pharmacology)
• Benzyl Compounds (pharmacology)
• Breast Neoplasms (enzymology, metabolism, pathology)
• Cell Adhesion (drug effects)
• Cell Line, Tumor
• Female
• Humans
• Matrix Metalloproteinase 2 (metabolism)
• Matrix Metalloproteinase 9 (metabolism)
• Mucin-1 (metabolism)
• Neoplasm Invasiveness