Effects of brefeldin A on the synthesis of chondroitin 4-sulfate by cultures of mouse mastocytoma cells.

Abstract

Mouse mastocytoma cells were cultured with brefeldin A in medium containing [35S]sulfate and [3H]glucosamine in order to determine the effects of this fungal metabolite on the formation of chondroitin 4-sulfate by these cells. There was a marked reduction in the incorporation of [35S]sulfate into the glycosaminoglycan which was approximately equal to the reduction in the incorporation of [3H]hexosamine into the same molecule. The chondroitin 4-sulfate chain size was greatly diminished, while the number of chains appeared to remain relatively constant, indicating that the brefeldin A partially disrupted the polymerizing system, but had little effect upon movement of the nascent proteochondroitin to the site for chondroitin polymerization and sulfation.

• Authors: G Sugumaran, M Katsman, J E Silbert
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 183 Issue 2 Pg. 357-61 (Mar 16 1992) ISSN: 0006-291X [Print] United States
• PMID: 1550544 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Cyclopentanes
• Mycotoxins
• Sulfates
• Brefeldin A
• Chondroitin Sulfates
• Glucosamine

Topics

• Animals
• Brefeldin A
• Chondroitin Sulfates (biosynthesis)
• Cyclopentanes (pharmacology)
• Glucosamine (metabolism)
• Mast-Cell Sarcoma (metabolism)
• Mice
• Mycotoxins (pharmacology)
• Sulfates (metabolism)
• Tumor Cells, Cultured