Abstract | PURPOSE: To determine abnormal meibomian gland secretion polar lipids from meibomianitis patients and to determine the significance of these lipid differences. METHODS: Meibomian gland secretions were collected from controls and patients with chronic blepharitis. These meibomian gland lipids (meibum) were first separated by thin-layer chromatography. Polar lipids were then separated utilizing high-pressure liquid chromatography with ultraviolet detection. Individual peaks were identified by comparison to standards and retention time. RESULTS: CONCLUSION: Many of the most significant differences in meibomianitis meibum composition occur in the polar lipids rather than the nonpolar lipids. Further investigations will determine the true significance of these findings.
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Authors | Ward E Shine, James P McCulley |
Journal | Cornea
(Cornea)
Vol. 23
Issue 8
Pg. 781-3
(Nov 2004)
ISSN: 0277-3740 [Print] United States |
PMID | 15502478
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Fatty Acids, Unsaturated
- Lysophosphatidylcholines
- Phosphatidylcholines
- Phospholipids
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Topics |
- Blepharitis
(metabolism)
- Chromatography, High Pressure Liquid
- Chromatography, Thin Layer
- Chronic Disease
- Fatty Acids, Unsaturated
(metabolism)
- Female
- Humans
- Lysophosphatidylcholines
(metabolism)
- Male
- Meibomian Glands
(metabolism)
- Middle Aged
- Phosphatidylcholines
(metabolism)
- Phospholipids
(metabolism)
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