Crystallization and preliminary X-ray crystallographic studies of mouse autocrine motility factor.

Abstract

Mouse autocrine motility factor (mAMF), a tumour-secreted cytokine that stimulates cell migration in vitro and metastasis in vivo, has been crystallized by the hanging-drop vapour-diffusion method. The crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 69.97, b = 115.88, c = 73.27 A, beta = 101.76 degrees . There are two subunits (one dimer) per asymmetric unit. Complexes with four-, five- and six-carbon carbohydrate phosphate inhibitors have also been crystallized. The crystals diffract to at least 1.8 A resolution and are suitable for X-ray structure analyses at high resolution.

• Authors: Noriko Naba, Nobutada Tanaka, Katsura Shiraiwa, Yoshio Kusakabe, Tatsuyoshi Funasaka, Arayo Haga, Hisamitsu Nagase, Avraham Raz, Kazuo T Nakamura
• Journal: Acta crystallographica. Section D, Biological crystallography (Acta Crystallogr D Biol Crystallogr) Vol. 60 Issue Pt 11 Pg. 2084-6 (Nov 2004) ISSN: 0907-4449 [Print] United States
• PMID: 15502335 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glucose-6-Phosphate Isomerase

Topics

• Animals
• Crystallization
• Crystallography, X-Ray
• Gene Expression
• Glucose-6-Phosphate Isomerase (chemistry, genetics, isolation & purification)
• Mice