Abstract | BACKGROUND: OBJECTIVES: METHODS: Patients with urethral condylomata (n = 164) were given topical ALA followed by intraurethral PDT through a cylindrical fibre. Patients included 11 individuals with 16 penile or vulval condylomatous lesions which were biopsied before or after treatment; the histological changes were then evaluated by light microscopy and electron microscopy. RESULTS: The complete response rate was 95% and the recurrence rate was 5% after 6-24 months of follow-up. Light microscopy revealed keratinocytes in the middle and upper layers of the epidermis showing marked vacuolation and some necrocytosis 1 and 3 h after PDT. Necrosis in all layers of the epidermis was noted 5 h after PDT. Electron microscopy of keratinocytes revealed distinct ultrastructural abnormalities of mitochondria and the endoplasmic reticulum, and membrane damage. Apoptotic bodies were detected 3 h after PDT and a large number of keratinocytes exhibited necrosis 5 h after PDT. CONCLUSIONS: Results suggest that, compared with conventional therapies, topical ALA- PDT is a simple, effective, safe and well-tolerated treatment for urethral condylomata acuminata that is associated with a low recurrence rate. The mechanism might be the triggering of both apoptosis and necrosis by ALA- PDT in human papillomavirus-infected keratinocytes.
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Authors | X L Wang, H W Wang, H S Wang, S Z Xu, K H Liao, P Hillemanns |
Journal | The British journal of dermatology
(Br J Dermatol)
Vol. 151
Issue 4
Pg. 880-5
(Oct 2004)
ISSN: 0007-0963 [Print] England |
PMID | 15491431
(Publication Type: Evaluation Study, Journal Article)
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Chemical References |
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Topics |
- Adult
- Aged
- Aminolevulinic Acid
(therapeutic use)
- Apoptosis
- Condylomata Acuminata
(drug therapy, pathology)
- Epidermis
(pathology)
- Feasibility Studies
- Female
- Humans
- Keratinocytes
(ultrastructure)
- Male
- Middle Aged
- Necrosis
- Penile Diseases
(drug therapy)
- Photochemotherapy
(methods)
- Recurrence
- Urethral Diseases
(drug therapy, pathology)
- Vulvar Diseases
(drug therapy)
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