Abstract | AIM: METHODS: The expression of Coxsackievirus B(3) RNA was detected in autopsy specimens of acute (12 cases), sub-acute (27 cases) and chronic (15 cases) Keshan disease by in situ hybridization. In sub-acute Keshan disease specimens, 3 cases with positive result by in situ hybridization were selected RT-PCR analysis. The DNA segments were then sequenced. RESULTS: Coxsackievirus B(3) RNA was detected in the cytoplasm of myocardiocyte. The positive rate was 83% in acute, 67% in sub-acute and 80% in chronic Keshan disease. In the conservative region of Coxsackievirus B(3) genome, there was a mutation in 234 (C-T) compared to the non-cardiovirulent strain, CVB(3/0). CONCLUSION: Coxsackievirus B(3) RNA can survive and replicate in heart muscle of Keshan disease, which may play an important role in the occurrence of Keshan disease. The possible mechanism of occurrence of Keshan disease is associated with point a mutation in Coxsackievirus B(3) genome.
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Authors | Li-Qun Ren, Xiang-Jun Li, Guang-Sheng Li, Zhi-Tao Zhao, Bo Sun, Fei Sun |
Journal | World journal of gastroenterology
(World J Gastroenterol)
Vol. 10
Issue 22
Pg. 3299-302
(Nov 15 2004)
ISSN: 1007-9327 [Print] United States |
PMID | 15484304
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- 5' Untranslated Regions
- RNA, Viral
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Topics |
- 5' Untranslated Regions
(genetics)
- Adolescent
- Adult
- Base Sequence
- Cardiomyopathy, Dilated
(virology)
- Child, Preschool
- Enterovirus B, Human
(genetics, pathogenicity)
- Enterovirus Infections
(virology)
- Female
- Humans
- In Situ Hybridization
- Male
- Molecular Sequence Data
- RNA, Viral
(analysis)
- Reverse Transcriptase Polymerase Chain Reaction
- Virulence
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