HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Coxsackievirus B3 infection and its mutation in Keshan disease.

AbstractAIM:
To investigate coxsackievirus B(3) infection and its gene mutation in Keshan disease.
METHODS:
The expression of Coxsackievirus B(3) RNA was detected in autopsy specimens of acute (12 cases), sub-acute (27 cases) and chronic (15 cases) Keshan disease by in situ hybridization. In sub-acute Keshan disease specimens, 3 cases with positive result by in situ hybridization were selected RT-PCR analysis. The DNA segments were then sequenced.
RESULTS:
Coxsackievirus B(3) RNA was detected in the cytoplasm of myocardiocyte. The positive rate was 83% in acute, 67% in sub-acute and 80% in chronic Keshan disease. In the conservative region of Coxsackievirus B(3) genome, there was a mutation in 234 (C-T) compared to the non-cardiovirulent strain, CVB(3/0).
CONCLUSION:
Coxsackievirus B(3) RNA can survive and replicate in heart muscle of Keshan disease, which may play an important role in the occurrence of Keshan disease. The possible mechanism of occurrence of Keshan disease is associated with point a mutation in Coxsackievirus B(3) genome.
AuthorsLi-Qun Ren, Xiang-Jun Li, Guang-Sheng Li, Zhi-Tao Zhao, Bo Sun, Fei Sun
JournalWorld journal of gastroenterology (World J Gastroenterol) Vol. 10 Issue 22 Pg. 3299-302 (Nov 15 2004) ISSN: 1007-9327 [Print] United States
PMID15484304 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • 5' Untranslated Regions
  • RNA, Viral
Topics
  • 5' Untranslated Regions (genetics)
  • Adolescent
  • Adult
  • Base Sequence
  • Cardiomyopathy, Dilated (virology)
  • Child, Preschool
  • Enterovirus B, Human (genetics, pathogenicity)
  • Enterovirus Infections (virology)
  • Female
  • Humans
  • In Situ Hybridization
  • Male
  • Molecular Sequence Data
  • RNA, Viral (analysis)
  • Reverse Transcriptase Polymerase Chain Reaction
  • Virulence

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: