TGF-beta differentially regulates TH2 cytokine-induced eotaxin and eotaxin-3 release by human airway smooth muscle cells.

Abstract

BACKGROUND:

Human airway smooth muscle cells (HASMs) are involved in the pathogenesis of asthma. By producing chemokines, HASMs play a role in the inflammatory processes observed in this disease. Eotaxin, eotaxin-2, and eotaxin-3 are important chemoattractants for eosinophils, and these chemokines are expressed during different phases of the allergic reaction. TH2 cytokines and TGF-beta can be found in increased levels in patients with asthma, and these cytokines may be involved in the regulation of chemokine expression.

OBJECTIVE:

The aim of this study was to determine the effect of TH2 cytokines and TGF-beta on the regulation of expression of eotaxin, eotaxin-2, and eotaxin-3 by HASMs.

METHODS:

HASMs were incubated for 24 hours with IL-4, IL-13, TGF-beta1, or combinations of these cytokines. Protein and mRNA levels of eotaxin and eotaxin-3 were evaluated by sandwich ELISA and reverse transcriptase-PCR.

RESULTS:

IL-4 and IL-13 induced mRNA and protein for both eotaxin and eotaxin-3. Eotaxin-2 mRNA and protein were not detected in HASMs. TGF-beta alone did not induce expression of the eotaxins. However, in combination with IL-4 or IL-13, TGF-beta enhanced eotaxin production and inhibited TH2 cytokine-induced eotaxin-3 production.

CONCLUSION:

TGF-beta differentially regulates TH2 cytokine-induced eotaxin and eotaxin-3 release.

• Authors: Suzanne Zuyderduyn, Pieter S Hiemstra, Klaus F Rabe
• Journal: The Journal of allergy and clinical immunology (J Allergy Clin Immunol) Vol. 114 Issue 4 Pg. 791-8 (Oct 2004) ISSN: 0091-6749 [Print] United States
• PMID: 15480317 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CCL11 protein, human
• CCL26 protein, human
• Chemokine CCL11
• Chemokine CCL26
• Chemokines, CC
• Interleukin-13
• Transforming Growth Factor beta
• Interleukin-4

Topics

• Bronchi (immunology)
• Chemokine CCL11
• Chemokine CCL26
• Chemokines, CC (immunology)
• Humans
• Interleukin-13 (immunology)
• Interleukin-4 (immunology)
• Myocytes, Smooth Muscle (immunology)
• Pneumonia (immunology)
• Th2 Cells (immunology)
• Transforming Growth Factor beta (immunology)