Antifibrotic and uveitogenic properties of gamma interferon in the rabbit eye.

Abstract

The feasibility of gamma (gamma)-interferon injection for the treatment of ocular fibrotic conditions was studied in rabbits using recombinant rabbit gamma-interferon and the cell-injection model of tractional detachment. A toxicity study revealed that intravitreal injection of greater than 10(4) units gamma-interferon consistently produced panuveitis. For tractional detachment, 250,000 rabbit dermal fibroblasts were injected intravitreally into 20 eyes; 1 day later, 5 of these eyes received intravitreal injections of 10(4) units gamma-interferon, another 5 were given 10(6) units, and the remaining 10 received balanced salt solution. Slit-lamp examination and fundus photography were performed at regular intervals for 21 days and were graded by a masked observer. The eyes were then enucleated and processed for histology. Doses of 10(4) units gamma-interferon significantly reduced the severity of detachments, but injections of 10(6) units induced panuveitis.

• Authors: L M Hjelmeland, J W Li, C A Toth, M B Landers 3rd
• Journal: Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie (Graefes Arch Clin Exp Ophthalmol) Vol. 230 Issue 1 Pg. 84-90 ( 1992) ISSN: 0721-832X [Print] Germany
• PMID: 1547974 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Recombinant Proteins
• Interferon-gamma

Topics

• Animals
• Cell Division (drug effects)
• Cells, Cultured
• Disease Models, Animal
• Eye Diseases (pathology)
• Fibroblasts (drug effects)
• Fibrosis
• Interferon-gamma (toxicity)
• Panuveitis (chemically induced, pathology)
• Rabbits
• Recombinant Proteins
• Retinal Detachment (pathology, prevention & control)
• Retinal Diseases (pathology)
• Vitreous Body (pathology)