Abstract |
Wistar and Buffalo rats of both sexes, aged 4 months, were divided into three groups: I which was given an intramuscular injection of 3 x 10(6) cells of Morris hepatoma (Buffalo males), II-- subcutaneous injection of 3 x 10(4) cells of mammary gland carcinoma (Wistar females), III-- intraperitoneal injection of 3 x 10(4) cells of Yoshid sarcoma (Wistar males). The animals were killed: in group I--19, group II--13 and in group III--6 days after tumor transplantation. Twenty four hours before euthanasia the rats were given 5-brome-2'-deoxyuridine (BRd-U) at a dose of 50 mg/kg body mass. The control group consisted of animals with tumour. They were not treated with BRd-U. Immunocytochemical reaction was performed on the sections of tumors, using monoclonal anti-BRd-U clone BU-33, Sigma. Computer measurements of tumor cells were carried out. There was a high similarity in morphological parameters between two kinds of cancer, and clear differences between them and Yoshid sarcoma. The main difference was noted in a twofold increase in the quantity of synthesised DNA in the nuclei of sarcoma cells. Immunocytochemical identification of tumor cells in phase S of the cell cycle with the use of monoclonal anti-BRd-U antibody is a precise and quick method of estimation of their proliferative potential.
|
Authors | M Nowak, J A Madej, S Dzimira, M Cegielski, I Całkosiński |
Journal | Polish journal of veterinary sciences
(Pol J Vet Sci)
Vol. 7
Issue 3
Pg. 163-70
( 2004)
ISSN: 1505-1773 [Print] Germany |
PMID | 15478860
(Publication Type: Evaluation Study, Journal Article)
|
Chemical References |
- Antibodies, Monoclonal
- Radiation-Sensitizing Agents
- Bromodeoxyuridine
|
Topics |
- Animals
- Antibodies, Monoclonal
(immunology)
- Bromodeoxyuridine
(immunology)
- Cell Cycle
- Clone Cells
- Female
- Immunohistochemistry
(methods)
- Liver Neoplasms, Experimental
(metabolism, pathology)
- Male
- Mammary Neoplasms, Experimental
(metabolism, pathology)
- Neoplasm Transplantation
- Neoplasms, Experimental
(metabolism, pathology)
- Radiation-Sensitizing Agents
- Rats
- Rats, Inbred BUF
- Rats, Wistar
- Sarcoma, Experimental
(metabolism, pathology)
|