HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Evaluation of a LAM ELISA for diagnosis of paratuberculosis in sheep and goats.

Abstract
A milk and serum ELISA containing lipoarabinomanan (LAM) antigen was evaluated in sheep and goats versus agar gel immunodiffusion (AGID) test and polymerase chain reaction (PCR) using milk and lymph nodes. Milk and serum samples were obtained from six, two, and four flocks with unknown, negative and positive status of infection, respectively. By comparison of serum ELISA activity and PCR results, the positive and negative predictive values were calculated. Receiver operation characteristic (ROC) analysis was used for calculating the specificity and sensitivity at different cut-offs.
AuthorsS K Munjal, J Boehmer, M Beyerbach, K Strutzberg-Minder, M Homuth
JournalVeterinary microbiology (Vet Microbiol) Vol. 103 Issue 1-2 Pg. 107-14 (Oct 05 2004) ISSN: 0378-1135 [Print] Netherlands
PMID15381273 (Publication Type: Comparative Study, Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Bacterial
  • DNA, Bacterial
  • Lipopolysaccharides
  • lipoarabinomannan
Topics
  • Animals
  • Antibodies, Bacterial (blood, immunology)
  • DNA, Bacterial (chemistry, genetics)
  • Enzyme-Linked Immunosorbent Assay (methods, veterinary)
  • Female
  • Goat Diseases (diagnosis, immunology, microbiology)
  • Goats
  • Immunodiffusion (veterinary)
  • Lipopolysaccharides (immunology)
  • Milk (microbiology)
  • Mycobacterium avium subsp. paratuberculosis (immunology, isolation & purification)
  • Paratuberculosis (diagnosis, immunology, microbiology)
  • Polymerase Chain Reaction (veterinary)
  • Predictive Value of Tests
  • Prevalence
  • Sensitivity and Specificity
  • Sheep
  • Sheep Diseases (diagnosis, immunology, microbiology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: