HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Effects of glycolic acid on the induction of apoptosis via caspase-3 activation in human leukemia cell line (HL-60).

Abstract
Apoptosis is a particular process that leads to the programmed cell death, and it has been a potentially therapeutic target of cancer. In this study, we evaluated the possible apoptotic effects of glycolic acid on human leukemia cell line (HL-60) in vitro. The morphological changes, cell viability, apoptosis induction, and caspase-3 activity were measured by phase microscopy, flow cytometry, and Western blot analysis. Morphological changes including shrinkage of cells were clearly demonstrated in HL-60 cells treated with increasing concentrations of glycolic acid. Cell viability was significantly affected by glycolic acid treatment in a dose- and time-dependent manner. In comparison to the control group, glycolic acid treatment had a profound effect in the induction of apoptosis by flow cytometric assays. In the cell cycle analysis, glycolic acid caused the increased percentage of cells in G2/M phase and the decreased expression of the cyclin A and cyclin B1, suggesting the induction of G2/M arrest of cell cycle by glycolic acid. Moreover, glycolic acid treatment promoted caspase-9 and -3 activity in a dose-dependent manner, but caspse-8 activity was not affected during the same process. Glycolic acid co-administrated with broad-spectrum caspase inhibitor, z-VAD-fmk, caspase-3 activity was blunted and apoptosis was also markedly blocked in HL-60 cells. In conclusion, glycolic acid-induced apoptosis in HL-60 cells may be through the activation of caspase-3. Future studies focusing on cell signaling and biological significance of glycolic acid-induced apoptosis would lead to exploring the mechanisms of chemotherapeutic potency of glycolic acid in human cancers.
AuthorsJen-Hung Yang, Chi-Chung Chou, Ya-Wen Cheng, Lee-Yan Sheen, Ming-Chi Chou, Hsin-Su Yu, Yau-Huei Wei, Jing-Gung Chung
JournalFood and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 42 Issue 11 Pg. 1777-84 (Nov 2004) ISSN: 0278-6915 [Print] England
PMID15350675 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Amino Acid Chloromethyl Ketones
  • Caspase Inhibitors
  • Cyclins
  • Cysteine Proteinase Inhibitors
  • Glycolates
  • Indicators and Reagents
  • benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone
  • glycolic acid
  • DNA
  • CASP3 protein, human
  • CASP9 protein, human
  • Caspase 3
  • Caspase 9
  • Caspases
Topics
  • Amino Acid Chloromethyl Ketones (pharmacology)
  • Analysis of Variance
  • Apoptosis (drug effects)
  • Blotting, Western
  • Caspase 3
  • Caspase 9
  • Caspase Inhibitors
  • Caspases (drug effects, metabolism)
  • Cell Cycle (drug effects)
  • Cell Survival (drug effects)
  • Cyclins (biosynthesis)
  • Cysteine Proteinase Inhibitors (pharmacology)
  • DNA (biosynthesis)
  • Dose-Response Relationship, Drug
  • Enzyme Activation (drug effects)
  • Flow Cytometry
  • Glycolates (pharmacology)
  • HL-60 Cells
  • Humans
  • Indicators and Reagents
  • Time Factors

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: