Abstract |
Herpes simplex virus type 1 (HSV-1) is a large (150-kb) double-stranded DNA virus that forms latent infections in neuronal cells of the human peripheral nervous system. Previous work determined that the HSV-1 genome is found in an ordered nucleosomal structure during latent infection. However, during lytic infection, it was unclear whether viral DNA was in a chromatin state. We examined HSV-1 during lytic infection using micrococcal nuclease digestion and chromatin immunoprecipitation. The HSV-1 genome is at least partially nucleosomal, although apparently not in a regular repeating structure. Analysis of histones associated with HSV-1, within both the promoter and the transcribed regions, revealed covalent amino tail modifications similar to those associated with active host mammalian genes. Certain of the modifications were detected in the temporal order expected of the immediate-early, early, and late gene classes. These data suggest that productive infection may be accompanied by acquisition of a permissive chromatin state.
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Authors | J R Kent, P-Y Zeng, D Atanasiu, J Gardner, N W Fraser, S L Berger |
Journal | Journal of virology
(J Virol)
Vol. 78
Issue 18
Pg. 10178-86
(Sep 2004)
ISSN: 0022-538X [Print] United States |
PMID | 15331750
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Copyright | Copyright 2004 American Society for Microbiology |
Chemical References |
- DNA, Viral
- Histones
- Nucleosomes
- Micrococcal Nuclease
- Lysine
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Topics |
- Base Sequence
- Cell Line
- DNA, Viral
(genetics, metabolism)
- Genome, Viral
- Herpesvirus 1, Human
(genetics, pathogenicity, physiology)
- Histones
(chemistry, genetics, metabolism)
- Humans
- Lysine
(chemistry)
- Methylation
- Micrococcal Nuclease
- Models, Biological
- Nucleosomes
(metabolism)
- Promoter Regions, Genetic
- Transcription, Genetic
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