[Fluorine-containing analog of diazoxide prevented apoptosis in neonatal cardiomyocytes during anoxia-reoxygenation].

Abstract

In experiments on the primary culture of isolated neonatal rat cardiomyocytes it was established that anoxia-reoxygenation activated the process of programmed cell death, apoptosis. The amount of apoptotic cells (defined by fragmentation of a nucleus with Hoechst 33342 staining) during anoxia-reoxygenation was increased in 2.1 fold (P < 0.05). The amount of living and necrotic cells was not changed significantly. Apoptosis of neonatal cardiomyocytes during anoxia-reoxygenation was prevented by activation of ATP-dependent potassium (K(ATP)) channels with diazoxide. Synthesized by us the fluorine-containing analogue of diazoxide had the similar effect: the amount of apoptotic cells was decreased to 3.7% that was similar to control meaning. Application of glybenclamide, which completely abrogated the action of diazoxide and its fluorine-containing analogue, allows us to assert that antiapoptotic effect of the substances mentioned above depends on K(ATP) channels opening.

• Authors: V S Nahibin, V Ie Dosenko, S M Pyvovar, O O Moĭbenko, L M Iahupol's'kyĭ
• Journal: Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994) (Fiziol Zh (1994)) Vol. 50 Issue 3 Pg. 3-8 ( 2004) ISSN: 2522-9028 [Print] Ukraine
• Vernacular Title: Ftorovanyĭ analoh diazoksydu poperedzhuie apoptoz neonatal'nykh kardiomiotsytiv pid chas anoksiï--reoksyhenatsiï.
• PMID: 15320423 (Publication Type: English Abstract, Journal Article)

Chemical References

• Membrane Proteins
• Potassium Channels
• mitochondrial K(ATP) channel
• Fluorine
• Diazoxide
• Oxygen

Topics

• Animals
• Animals, Newborn
• Apoptosis (drug effects)
• Cell Hypoxia
• Cells, Cultured
• Diazoxide (analogs & derivatives, pharmacology)
• Fluorine (chemistry)
• Membrane Proteins (metabolism)
• Models, Biological
• Myocytes, Cardiac (drug effects, metabolism)
• Oxygen (pharmacology)
• Potassium Channels
• Rats