[Use of polymerase chain reaction in the diagnosis of tuberculosis in children with diseases of the bronchopulmonary system].

Abstract

A comprehensive study was conducted to determine the activity of tuberculous infection in 115 patients who formed 3 groups: 1) patients with primary forms of tuberculosis; 2) those who were infected with Mycobacterium tuberculosis (MBT); and 3) those who were infected with bronchopulmonary diseases. The detection of MBT DNA in the blood by polymerase chain reaction (PCR) using a highly sensitive four-primed diagnostic kit established that 80 patients with tuberculosis had positive results and their decrease as the patient had a course of tuberculostatic therapy. The fact that a third of patients from Groups 2 and 3 had highly positive results of PCR required that a preventive course of double therapy be used for 3 to 6 months.

• Authors: L V Klochkova, O I Korol', I A Putintseva, N A Skobeleva, O A Vostriukhina
• Journal: Problemy tuberkuleza i boleznei legkikh (Probl Tuberk Bolezn Legk) Issue 6 Pg. 49-51 ( 2004) ISSN: 1728-2993 [Print] Russia (Federation)
• Vernacular Title: Ispol'zovanie polimeraznoĭ tsepnoĭ reaktsii dlia diagnostiki tuberkuleza u deteĭ s patologieĭ bronkholegochnoĭ sistemy.
• PMID: 15315134 (Publication Type: English Abstract, Journal Article)

Topics

• Adolescent
• Bronchi (physiopathology)
• Child
• Diagnosis, Differential
• Humans
• Lung (physiopathology)
• Polymerase Chain Reaction (methods)
• Tuberculosis, Pulmonary (diagnosis, physiopathology)