Abstract | OBJECTIVE: METHODS: The exogenous wild PTEN cDNA via an adenoviral vector (Ad-PTEN) was introduced into Ishikawa cells. The expression of PTEN protein was detected by Western blot. The growth of Ishikawa cells was evaluated by trypan blue exclusion method and MTT. RESULTS: The expression of PTEN protein was induced on day 1, and greatly increasing on day 3 - 5 after Ad-PTEN infection. The expression of PTEN significantly inhibited the growth of Ishikawa cells, and also significantly inhibited the growth of Ishikawa cells induced by IGF-II. CONCLUSION:
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Authors | Xiao-yun Wan, Yi-fu Shi, Juan-qing Li |
Journal | Zhonghua zhong liu za zhi [Chinese journal of oncology]
(Zhonghua Zhong Liu Za Zhi)
Vol. 26
Issue 5
Pg. 275-8
(May 2004)
ISSN: 0253-3766 [Print] China |
PMID | 15312361
(Publication Type: Journal Article)
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Chemical References |
- Tumor Suppressor Proteins
- Insulin-Like Growth Factor II
- Phosphoric Monoester Hydrolases
- PTEN Phosphohydrolase
- PTEN protein, human
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Topics |
- Adenoviridae
(genetics)
- Cell Proliferation
- Endometrial Neoplasms
(metabolism, pathology)
- Female
- Gene Expression Regulation, Neoplastic
- Humans
- Insulin-Like Growth Factor II
(pharmacology)
- PTEN Phosphohydrolase
- Phosphoric Monoester Hydrolases
(biosynthesis, genetics, physiology)
- Recombination, Genetic
- Transfection
- Tumor Suppressor Proteins
(biosynthesis, genetics, physiology)
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