Abstract | AIM: METHODS: Hydrophobic protein samples were extracted from normal colorectal mucosa, primary cancer lesion and hepatic metastatic foci of colorectal cancer. With two-dimensional electrophoresis and image analysis, differentially expressed protein spots were detected, and the proteins were identified by matrix assisted laser desorption/ionization-time of flight-mass spectrometry and peptide mass fingerprint analysis. RESULTS: Significant alterations of the proteins in number and expression levels were discovered in primary cancer and hepatic metastatic foci, the expression of a number of proteins was lost in 25-40 ku, but protein spots was increased in 14-21 ku, compared with normal mucosa. Nine differentially expressed protein spots were identified. Three proteins expressed in normal mucosa, but lost in primary cancer and hepatic metastasis, were recognized as calmodulin, ribonuclease 6 precursor and mannosidase-alpha. Proapolipoprotein was expressed progressively from normal mucosa to primary cancer and hepatic metastasis. The differentially expressed protein of beta-globin was found in normal mucosa and hepatic metastatic tumor, but lost in primary cancer lesion. Cdc 42, a GTP-binding protein, was identified in hepatic metastasis. The protein spots of C4 from primary cancer, M7 and M9 from hepatic metastasis had less homology with the proteins in database. CONCLUSION:
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Authors | Bo Yu, Shi-Yong Li, Ping An, Ying-Nan Zhang, Zhen-Jia Liang, Shu-Jun Yuan, Hui-Yun Cai |
Journal | World journal of gastroenterology
(World J Gastroenterol)
Vol. 10
Issue 18
Pg. 2652-6
(Sep 15 2004)
ISSN: 1007-9327 [Print] United States |
PMID | 15309713
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Adult
- Aged
- Colonic Neoplasms
(chemistry, pathology)
- Electrophoresis, Gel, Two-Dimensional
- Female
- Humans
- Liver Neoplasms
(chemistry, secondary)
- Male
- Middle Aged
- Peptide Mapping
- Proteome
(analysis)
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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