Abstract | OBJECTIVE: To investigate the frequency of microsatellite instability (MSI) and loss of heterozygosity (LOH) at human leucocyte antigen(HLA) class I loci in cervical carcinoma and the detailed deletion mapping in this region. METHODS: LOH and MSI of HLA class I genes were analyzed in 30 paired blood and tumor samples by PCR based single-stranded length polymorphism (PCR-SSLP). RESULTS: Of the 30 cases, 23(76.7%) showed LOH at one or more loci. Higher frequencies of LOH were found at four loci: C3_2_11 (50%), C1_4_4 (37%), C1_2_5 (36.7%), D6S276 (48.3%). MSI was found in 20 out of 30 cases (66.7%). CONCLUSION: The data suggest that the LOH and MSI of HLA class I gene might participate in the carcinogenesis of cervical carcinoma. Meanwhile, the minimal deletion region might be defined between C1_2_5 and C3_2_11, thus providing the evidence for cloning the tumor suppressor genes associated with cervical carcinoma.
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Authors | Yuan Rong, Xu-feng Wu, Guan-min Chen, Min Zhao, Xin-xing Wu |
Journal | Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics
(Zhonghua Yi Xue Yi Chuan Xue Za Zhi)
Vol. 21
Issue 4
Pg. 342-6
(Aug 2004)
ISSN: 1003-9406 [Print] China |
PMID | 15300630
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Histocompatibility Antigens Class I
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Topics |
- Adult
- Aged
- Female
- Histocompatibility Antigens Class I
(genetics)
- Humans
- Loss of Heterozygosity
(genetics)
- Microsatellite Instability
- Middle Aged
- Polymerase Chain Reaction
- Uterine Cervical Neoplasms
(genetics)
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