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[Microsatellite instability and loss of heterozygosity of human leucocyte antigen class I gene in cervical carcinoma].

AbstractOBJECTIVE:
To investigate the frequency of microsatellite instability (MSI) and loss of heterozygosity (LOH) at human leucocyte antigen(HLA) class I loci in cervical carcinoma and the detailed deletion mapping in this region.
METHODS:
LOH and MSI of HLA class I genes were analyzed in 30 paired blood and tumor samples by PCR based single-stranded length polymorphism (PCR-SSLP).
RESULTS:
Of the 30 cases, 23(76.7%) showed LOH at one or more loci. Higher frequencies of LOH were found at four loci: C3_2_11 (50%), C1_4_4 (37%), C1_2_5 (36.7%), D6S276 (48.3%). MSI was found in 20 out of 30 cases (66.7%).
CONCLUSION:
The data suggest that the LOH and MSI of HLA class I gene might participate in the carcinogenesis of cervical carcinoma. Meanwhile, the minimal deletion region might be defined between C1_2_5 and C3_2_11, thus providing the evidence for cloning the tumor suppressor genes associated with cervical carcinoma.
AuthorsYuan Rong, Xu-feng Wu, Guan-min Chen, Min Zhao, Xin-xing Wu
JournalZhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics (Zhonghua Yi Xue Yi Chuan Xue Za Zhi) Vol. 21 Issue 4 Pg. 342-6 (Aug 2004) ISSN: 1003-9406 [Print] China
PMID15300630 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Histocompatibility Antigens Class I
Topics
  • Adult
  • Aged
  • Female
  • Histocompatibility Antigens Class I (genetics)
  • Humans
  • Loss of Heterozygosity (genetics)
  • Microsatellite Instability
  • Middle Aged
  • Polymerase Chain Reaction
  • Uterine Cervical Neoplasms (genetics)

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