Immunohistochemical staining of cutaneous tumours with G-81, a monoclonal antibody to dermcidin.

Abstract

BACKGROUND:

Recently, the novel antimicrobial peptide named dermcidin (DCD) was reported in human eccrine sweat glands.

OBJECTIVES:

We investigated the expression of DCD in a variety of cutaneous tumours in order to assess the usefulness of the monoclonal antibody (G-81), which recognizes a fragment of DCD.

PATIENTS/METHODS:

We studied the immunoreactivity of the G-81 antibody on 197 cutaneous tumours.

RESULTS:

A total of 13 of 26 cutaneous mixed tumours showed substantial immunoreactivity. In contrast all the following cases were completely unreactive: (i) epithelial tumours (seborrhoeic keratosis, squamous cell carcinoma, Bowen's disease, actinic keratosis, genital Paget's disease); (ii) follicular tumours (basal cell carcinoma, trichilemmoma, trichoepithelioma, trichoblastoma, keratoacanthoma, proliferating trichilemmal tumour, pilomatricoma); (iii) melanocytic tumours (malignant melanoma, naevus cell naevus, Spitz naevus, blue naevus); (iv) neural tumours (schwannoma, neurofibroma, Merkel cell neoplasm); (v) mesenchymal tumours (soft fibroma, dermatofibroma, dermatofibrosarcoma protuberans, vascular leiomyoma, leiomyosarcoma, lipoma, juvenile xanthogranuloma, angiomyoma); and (vi) other sweat gland tumours (poroid neoplasms, syringoma, cylindroma, clear cell hidradenoma, spiradenoma, syringoid eccrine carcinoma, mucinous carcinoma, apocrine cystadenoma, syringocystadenoma papilliferum, apocrine adenocarcinoma). Twenty-six cutaneous mixed tumours were considered from histopathological findings to be the apocrine type, but 13 of 26 mixed tumours contained some DCD-immunopositive cells that possibly differentiate into eccrine secretory glands.

CONCLUSIONS:

We found the expression of DCD in tubular structures of 50% of cutaneous mixed tumours with apocrine differentiation. These results suggest that a number of cutaneous mixed tumours show both eccrine and apocrine differentiation in the same neoplasm.

• Authors: Y Minami, K Uede, K Sagawa, A Kimura, T Tsuji, F Furukawa
• Journal: The British journal of dermatology (Br J Dermatol) Vol. 151 Issue 1 Pg. 165-9 (Jul 2004) ISSN: 0007-0963 [Print] England
• PMID: 15270886 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Peptides
• dermcidin

Topics

• Antibodies, Monoclonal
• Humans
• Immunohistochemistry (methods)
• Peptides (immunology)
• Predictive Value of Tests
• Skin Neoplasms (diagnosis, pathology)
• Sweat Gland Neoplasms (diagnosis, pathology)