Successful sentinel node identification in colon carcinoma using Patent Blue V.

Abstract

AIM:

The aim of this study was to evaluate the use of Patent Blue V for identification of the sentinel node in patients with colon carcinoma.

METHOD:

From May 2002, 35 patients operated for colon carcinoma underwent lymphatic mapping using Patent Blue V as marker. Either directly after resection of the colon or during operation 2 ml of Patent Blue V was injected peritumourally, and the first 1 to 4 blue nodes were marked as sentinel nodes. Pathological evaluation was done on a single HE-stained section of all lymph nodes. Only if all sentinel nodes were negative for metastases, serial sectioning and additional immunohistochemical staining against keratine CK 7/8 was performed to reveal micrometastasis in the sentinel nodes.

RESULTS:

In 33/35 of patients at least one sentinel node was identified. In 10/33 the sentinel node was positive for metastases, and in 5/10 this was the only node containing metastases. One patient had a false negative sentinel node (accuracy 97%, sensitivity 91%).

CONCLUSION:

Using Patent Blue V, it is possible to identify the sentinel node in most patients with colon cancer. The results are comparable with other sentinel node studies using Lymphazurin.

• Authors: A E Braat, J W A Oosterhuis, F C P Moll, J E de Vries
• Journal: European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology (Eur J Surg Oncol) Vol. 30 Issue 6 Pg. 633-7 (Aug 2004) ISSN: 0748-7983 [Print] England
• PMID: 15256237 (Publication Type: Journal Article)

Chemical References

• Coloring Agents
• Rosaniline Dyes
• sulfan blue

Topics

• Colectomy (methods)
• Colonic Neoplasms (pathology, surgery)
• Coloring Agents
• Humans
• Lymphatic Metastasis
• Neoplasm Staging
• Rosaniline Dyes
• Sentinel Lymph Node Biopsy (methods)
• Treatment Outcome