Detection of circulating antigens in experimental anisakiasis by two-site enzyme-linked immunosorbent assay.

Abstract

A two-site enzyme-linked immunosorbent assay (ELISA) based on the avidin-biotin system was developed to study circulating antigens in the sera of rats orally or intraperitoneally infected with L3 larvae of Anisakis simplex s.l. Somatic and excretory-secretory antigens were detected from 24 h post-infection in all infected rats. A direct relationship between the number of larvae inoculated and the concentration of antigens in the sera was demonstrated; however, the amount of antigen decreased with the course of the infection. Comparative analysis revealed that intraperitoneal inoculation is most appropriate for detecting excretory-secretory antigens in serum. The minimum concentrations of A. simplex antigen detectable were approximately 2.5 microg/ml for somatic and excretory-secretory antigens. This antigen detection assay may have application in the diagnosis of anisakiasis.

• Authors: M Campos, L Martín, V Díaz, I Mañas, B Morales, J Lozano
• Journal: Parasitology research (Parasitol Res) Vol. 93 Issue 6 Pg. 433-8 (Aug 2004) ISSN: 0932-0113 [Print] Germany
• PMID: 15221461 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Helminth

Topics

• Animals
• Anisakiasis (diagnosis, parasitology)
• Anisakis (immunology)
• Antigens, Helminth (blood)
• Enzyme-Linked Immunosorbent Assay (methods, statistics & numerical data)
• Larva (immunology)
• Male
• Rats
• Rats, Wistar
• Sensitivity and Specificity