Generation of analytic plasma lipoprotein profiles using two prepacked superose 6B columns.

Abstract	A simple, low-priced chromatographic system to generate plasma lipoprotein profiles from total human plasma was tested with plasma from normolipidemic subjects and patients with heterozygous familial hypercholesterolemia, hyper-alpha-lipoproteinemia, lipoprotein lipase deficiency, familial dysbetalipoproteinemia and familial lecithin-cholesterol-acyl-transferase deficiency. The system appears to be a good alternative to more expensive high-pressure liquid chromatography systems, notably in lipoprotein laboratories already provided with equipment for column chromatography. If a microplate photometer and a computer is available in the laboratory, the measurement of various lipids in 70-80 eluant fractions from the columns can be simplified.
Authors	L U Gerdes, C Gerdes, I C Klausen, O Faergeman
Journal	Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 205 Issue 1-2 Pg. 1-9 (Jan 31 1992) ISSN: 0009-8981 [Print] Netherlands
PMID	1521329 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Lipoproteins Lipoproteins, HDL Lipoproteins, LDL Lipoproteins, VLDL Triglycerides Superose 6b Sepharose Cholesterol
Topics	Adult Cholesterol (blood) Chromatography, Liquid (methods) Female Humans Hyperlipidemias (blood) Lipoproteins (blood) Lipoproteins, HDL (blood) Lipoproteins, LDL (blood) Lipoproteins, VLDL (blood) Male Middle Aged Reproducibility of Results Sepharose Triglycerides (blood)

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