Progelatinase B/matrix metalloproteinase-9 proenzyme as a marker of pleural inflammation.

Abstract

Previous analyses of pleural effusions of different origin revealed high levels of matrix metalloproteinase-9 proenzyme (proMMP-9), predominantly in parainfectious exudates. To confirm the etiological association with inflammation and to explore time course of release of proMMP-9 under conditions of a local inflammatory reaction, the authors estimated proMMP-9 in pleural fluids of patients undergoing therapeutic talc pleurodesis (n = 20) and explorative thoracoscopy (n = 4), before, and at different time intervals after the intervention. The authors also compared proMMP-9 values with proinflammatory markers, such as interleukin-6 (IL-6), C-reactive protein (CRP), and leukocyte count. As revealed through scanning substrate electrophoresis, proMMP-9 levels increased within the first hours a!nd culminated 16 to 48 hours after intervention, amounting to 1.5- to 102 -fold of initial concentration (mean = 14.8 -fold). MMP-9 proenzyme values significantly correlated with the levels of proinflammatory cytokine IL-6 (r =.79, n = 47, p <.01) and with neutrophil counts (r =.75, n = 45, p <.01). In conclusion, the results present proMMP-9 as a novel early indicator of acute host-response in pleural space that complements traditional proinflammatory markers and might be useful in monitoring pleural inflammatory processes.

• Authors: Jaromír Kotyza, Milos Pesek, Petr Puzman, David Havel
• Journal: Experimental lung research (Exp Lung Res) Vol. 30 Issue 4 Pg. 297-309 (Jun 2004) ISSN: 0190-2148 [Print] England
• PMID: 15204835 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• Enzyme Precursors
• Interleukin-6
• Talc
• C-Reactive Protein
• L-Lactate Dehydrogenase
• Gelatinases
• Metalloendopeptidases
• progelatinase
• Matrix Metalloproteinase 9

Topics

• Aged
• Biomarkers (analysis)
• C-Reactive Protein (metabolism)
• Enzyme Precursors (metabolism)
• Female
• Gelatinases (metabolism)
• Humans
• Inflammation (enzymology)
• Interleukin-6 (metabolism)
• L-Lactate Dehydrogenase (metabolism)
• Leukocyte Count
• Male
• Matrix Metalloproteinase 9 (metabolism)
• Metalloendopeptidases (metabolism)
• Middle Aged
• Pleura (enzymology, pathology)
• Pleural Effusion (cytology)
• Pleurodesis (adverse effects)
• Talc (administration & dosage, pharmacology, therapeutic use)
• Thoracoscopy (adverse effects)
• Treatment Outcome