Screening of a HUAEC cDNA library identifies actin as a candidate autoantigen associated with carotid atherosclerosis.
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| Abstract |
The humoral immune response to endothelium has a pivotal role in the development of atherosclerosis. Using a molecular method, we sought to identify endothelial autoantigens in carotid atherosclerosis. Immunoscreening of a HUAEC expression library with IgG from a pool of two sera from patients with carotid atherosclerosis identified a clone specific to actin. We evaluated actin-specific IgG reactivity in patients with carotid atherosclerosis and compared responses with those in patients with systemic lupus erythematosus and type 1 diabetes mellitus and in healthy subjects. Enzyme-linked immunoassay detected actin-specific IgG in a significantly higher percentage of sera from patients with atherosclerosis and systemic lupus erythematosus than from healthy subjects (16/61, 26% and 13/33, 39%versus 2/41, 5%, P = 0.012 and P < 10(-4), by chi2 test). Mean optical density values were significantly higher in patients than in healthy subjects (P < 10(-4) by Student's t-test). Patients with atherosclerosis and uncomplicated plaques had significantly higher serum anti-actin IgG reactivity than those with complicated plaques (P = 0.048 by Student's t-test). Our findings suggest that actin is an autoantigenic molecule of potential clinical interest in carotid atherosclerosis.
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| Authors | P Margutti, F Delunardo, M Sorice, G Valesini, C Alessandri, R Capoano, E Profumo, A Siracusano, B Salvati, R Riganò, E Ortona |
| Journal | Clinical and experimental immunology (Clin Exp Immunol) Vol. 137 Issue 1 Pg. 209-15 (Jul 2004) ISSN: 0009-9104 [Print] England |
| PMID | 15196264 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't) |
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