Influence of Serum Amyloid A (SAA1) and SAA2 gene polymorphisms on renal amyloidosis, and on SAA/C-reactive protein values in patients with familial mediterranean fever in the Turkish population.

Abstract

OBJECTIVE:

To evaluate the effect of serum amyloid A (SAA) 1 and SAA2 gene polymorphisms on SAA levels and renal amyloidosis in Turkish patients with familial Mediterranean fever (FMF).

METHODS:

SAA1 and SAA2 gene polymorphisms and SAA levels were determined in 74 patients with FMF (39 female, 35 male; median age 11.5 yrs, range 1.0-23.0). All patients were on colchicine therapy. SAA1 and SAA2 gene polymorphisms were analyzed using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). SAA and C-reactive protein (CRP) values were measured and SAA/CRP values were calculated.

RESULTS:

The median SAA level was 75 ng/ml (range 10.2-1500). SAA1 gene polymorphisms were: a/a genotype in 23 patients (31.1%), a/beta genotype in 30 patients (40.5%), a/g genotype in one patient (1.4 %), beta/beta genotype in 14 patients (18.9%), beta/g genotype in 5 patients (6.8 %), and g/g genotype in one patient (1.4%). Of the 23 patients who had a/a genotype for the SAA1 polymorphism, 7 patients had developed renal amyloidosis (30.4%) compared to only one patient without this genotype (1/51; 2.0%); p < 0.001. SAA2 had no effect on renal amyloidosis. SAA1 and SAA2 genotypes had no significant effect on SAA levels. SAA/CRP values were significantly lower in patients with the SAA1a/a genotype, compared to other SAA1 genotypes: 0.16 (0.025-1.96) versus 0.23 (0.012-28.20), p < 0.05.

CONCLUSION:

SAA1a/a genotype is one genetic factor that confers a significant risk for amyloidosis in the Turkish FMF population. Neither the SAA1 nor SAA2 genotypes had a significant effect on SAA level.

• Authors: Aysin Bakkaloglu, Ali Duzova, Seza Ozen, Banu Balci, Nesrin Besbas, Rezan Topaloglu, Fatih Ozaltin, Engin Yilmaz
• Journal: The Journal of rheumatology (J Rheumatol) Vol. 31 Issue 6 Pg. 1139-42 (Jun 2004) ISSN: 0315-162X [Print] Canada
• PMID: 15170927 (Publication Type: Journal Article)

Chemical References

• SAA2 protein, human
• Serum Amyloid A Protein
• C-Reactive Protein

Topics

• Adolescent
• Adult
• Amyloidosis (genetics, immunology, pathology)
• C-Reactive Protein (metabolism)
• Child
• Child, Preschool
• Familial Mediterranean Fever (genetics, immunology, pathology)
• Female
• Genetic Predisposition to Disease
• Genotype
• Humans
• Infant
• Kidney (immunology, pathology)
• Male
• Polymorphism, Genetic
• Serum Amyloid A Protein (genetics)
• Turkey