Abstract |
We have identified that StarD10, a member of the START protein family, is overexpressed in both mouse and human breast tumors. StarD10 was initially discovered on the basis of its cross-reactivity with a phosphoserine-specific antibody in mammary tumors from Neu/ErbB2 transgenic mice and subsequently isolated from SKBR3 human breast carcinoma cells using a multistep biochemical purification strategy. We have shown that StarD10 is capable of binding lipids. StarD10 was found to be overexpressed in 35% of primary breast carcinomas and 64% of human breast cancer cell lines, correlating with their ErbB2/Her2 status. Coexpression of StarD10 with ErbB1/ epidermal growth factor receptor in murine fibroblasts enhanced anchorage-independent growth in soft agar, providing evidence for functional cooperation between StarD10 and ErbB receptor signaling. Taken together, these data suggest that overexpression of this lipid- binding protein contributes to breast oncogenesis.
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Authors | Monilola A Olayioye, Peter Hoffmann, Thomas Pomorski, Jane Armes, Richard J Simpson, Bruce E Kemp, Geoffrey J Lindeman, Jane E Visvader |
Journal | Cancer research
(Cancer Res)
Vol. 64
Issue 10
Pg. 3538-44
(May 15 2004)
ISSN: 0008-5472 [Print] United States |
PMID | 15150109
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Forkhead Box Protein O1
- Forkhead Transcription Factors
- Foxo1 protein, mouse
- Phosphoproteins
- STARD10 protein, human
- Stard10 protein, mouse
- Transcription Factors
- ErbB Receptors
- Receptor, ErbB-2
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Topics |
- Amino Acid Sequence
- Animals
- Antibody Specificity
- Breast Neoplasms
(metabolism, pathology)
- Cell Line, Tumor
- Cell Transformation, Neoplastic
(metabolism, pathology)
- Cross Reactions
- ErbB Receptors
(biosynthesis, metabolism)
- Forkhead Box Protein O1
- Forkhead Transcription Factors
- Humans
- Mammary Neoplasms, Experimental
(metabolism, pathology)
- Mice
- Molecular Sequence Data
- Molecular Weight
- Phosphoproteins
(biosynthesis, chemistry, metabolism)
- Receptor, ErbB-2
(biosynthesis, metabolism)
- Sequence Homology, Amino Acid
- Transcription Factors
(chemistry, immunology)
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