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In vivo and in vitro analysis of the resistance against viral haemorrhagic septicaemia virus in Japanese flounder (Paralichthys olivaceus) precedingly infected with aquabirnavirus.

Abstract
The resistance of Japanese flounder (Paralichthys olivaceus Temminck et Schlegel) against a viral haemorrhagic septicaemia virus (VHSV) challenge induced by a preceding non-lethal aquabirnavirus (ABV) challenge was investigated through experimental dual-infections with different intervals between the two challenges. The non-specific protection conferred by the primary ABV infection against the secondary VHSV infection commenced at Day 3 and persisted up to Day 14 but vanished at Day 21 post-ABV challenge. The in vitro assay using HINAE (hirame natural embryo) cells demonstrated anti-VHSV activity in the serum of ABV-challenged flounder from Day 1 to Day 14 but not at Day 21 post-ABV challenge. A high expression of a Mx gene, a molecular marker of type I interferon(s) (IFN) occurred in the head kidneys of ABV-challenged flounder from Day 1 to Day 7. These results suggest that the non-specific protection against the secondary VHSV infection in flounder was due to IFN(s) induced by the primary ABV infection.
AuthorsRolando Pakingking Jr, Yasushi Okinaka, Koh-Ichiro Mori, Misao Arimoto, Kiyokuni Muroga, Toshihiro Nakai
JournalFish & shellfish immunology (Fish Shellfish Immunol) Vol. 17 Issue 1 Pg. 1-11 (Jul 2004) ISSN: 1050-4648 [Print] England
PMID15145413 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Myxovirus Resistance Proteins
  • Interferons
  • GTP-Binding Proteins
Topics
  • Animals
  • Aquabirnavirus (immunology)
  • Enzyme-Linked Immunosorbent Assay
  • Flounder
  • GTP-Binding Proteins (genetics, metabolism)
  • Gene Expression
  • Hemorrhagic Septicemia, Viral (immunology)
  • Immunity, Innate
  • Interferons (blood)
  • Kidney (metabolism, virology)
  • Kinetics
  • Myxovirus Resistance Proteins
  • Novirhabdovirus (immunology)
  • Time Factors

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