Abstract |
The resistance of Japanese flounder (Paralichthys olivaceus Temminck et Schlegel) against a viral haemorrhagic septicaemia virus (VHSV) challenge induced by a preceding non-lethal aquabirnavirus (ABV) challenge was investigated through experimental dual- infections with different intervals between the two challenges. The non-specific protection conferred by the primary ABV infection against the secondary VHSV infection commenced at Day 3 and persisted up to Day 14 but vanished at Day 21 post-ABV challenge. The in vitro assay using HINAE (hirame natural embryo) cells demonstrated anti-VHSV activity in the serum of ABV-challenged flounder from Day 1 to Day 14 but not at Day 21 post-ABV challenge. A high expression of a Mx gene, a molecular marker of type I interferon(s) (IFN) occurred in the head kidneys of ABV-challenged flounder from Day 1 to Day 7. These results suggest that the non-specific protection against the secondary VHSV infection in flounder was due to IFN(s) induced by the primary ABV infection.
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Authors | Rolando Pakingking Jr, Yasushi Okinaka, Koh-Ichiro Mori, Misao Arimoto, Kiyokuni Muroga, Toshihiro Nakai |
Journal | Fish & shellfish immunology
(Fish Shellfish Immunol)
Vol. 17
Issue 1
Pg. 1-11
(Jul 2004)
ISSN: 1050-4648 [Print] England |
PMID | 15145413
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Myxovirus Resistance Proteins
- Interferons
- GTP-Binding Proteins
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Topics |
- Animals
- Aquabirnavirus
(immunology)
- Enzyme-Linked Immunosorbent Assay
- Flounder
- GTP-Binding Proteins
(genetics, metabolism)
- Gene Expression
- Hemorrhagic Septicemia, Viral
(immunology)
- Immunity, Innate
- Interferons
(blood)
- Kidney
(metabolism, virology)
- Kinetics
- Myxovirus Resistance Proteins
- Novirhabdovirus
(immunology)
- Time Factors
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