Propylene is a major industrial intermediate and atmospheric
pollutant to which humans are exposed by inhalation. In this study, 6-week-old male F344 rates were exposed to 0, 200, 2000, or 10,000 ppm
propylene by inhalation for 4 weeks (6 h/day, 5 days/week), and mutant frequencies were determined in the
Hprt gene of splenic T-lymphocytes. Twenty milligrams of
cyclophosphamide monohydrate (
CPP)/kg bw, given on the penultimate day of
propylene exposure, was used as a positive control
mutagen. Rats (n = 8/group) were necropsied for isolation of T-cells 8 weeks after the last dose, a sampling time that produced peak spleen
Hprt mutant frequencies (Mfs) in a preliminary mutant manifestation study using CCP treatment.
Hprt Mfs were measured via the T-cell cloning assay, which was performed without knowledge of the animal treatment groups. Mean
Hprt Mfs were significantly increased over control values (mean Mf = 5.24 +/- 1.55 (SD) x 10(-6)) in
CPP-treated rats (10.37 +/- 4.30 x 10(-6), P = 0.007). However,
Hprt Mfs in
propylene-exposed rats were not significantly increased over background, with mean Mfs of 4.90 +/- 1.84 x 10(-6) (P = 0.152), 5.05 +/- 3.70 x 10(-6) (P = 0.895), and 5.95 +/- 2.49 x10(-6) (P = 0.500) for animals exposed to 200, 2000, or 10,000 ppm
propylene, respectively. No significant increase in F344 rat or B6C3F1 mouse
cancer incidence was reported in the National Toxicology Program carcinogenicity studies of
propylene across this same exposure range. Taken together, these findings support the conclusion that inhalation exposure of rats to
propylene does not cause mutations or
cancer.