Abstract |
Mouse adenovirus type 1 (MAV-1) infection of B-cell-deficient and Bruton's tyrosine kinase (Btk)-deficient mice resulted in fatal disseminated disease resembling human adenovirus infections in immunocompromised patients. Mice lacking B cells or Btk were highly susceptible to acute MAV-1 infection, in contrast to controls and mice lacking T cells. To our knowledge, this is the first demonstration that mice with an X-linked immunodeficiency phenotype (Btk deficient) are susceptible to virus-induced disease. Mice lacking B cells or Btk on a C57BL/6 background succumbed with encephalomyelitis, hepatitis, and lymphoid necrosis. Mice lacking B cells on a BALB/c background succumbed with enteritis and hepatitis. Survival of acute MAV-1 infection correlated with early T-cell-independent neutralizing antibody and T-cell-independent antiviral immunoglobulin M. Treatment of MAV-1-infected Btk(-/-) mice 4 to 9 days postinfection with antiserum harvested 6 to 9 days postinfection from MAV-1-infected Btk(+/+) mice was therapeutic. Our findings implicate a critical role for B-cell function in preventing disseminated MAV-1 infection, particularly production of early T-cell-independent antiviral immunoglobulin M.
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Authors | Martin L Moore, Erin L McKissic, Corrie C Brown, John E Wilkinson, Katherine R Spindler |
Journal | Journal of virology
(J Virol)
Vol. 78
Issue 11
Pg. 5584-90
(Jun 2004)
ISSN: 0022-538X [Print] United States |
PMID | 15140955
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- FASLG protein, human
- Fas Ligand Protein
- Fasl protein, mouse
- Membrane Glycoproteins
- RNA, Messenger
- Protein-Tyrosine Kinases
- Agammaglobulinaemia Tyrosine Kinase
- BTK protein, human
- Btk protein, mouse
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Topics |
- Adenoviridae Infections
(immunology)
- Agammaglobulinaemia Tyrosine Kinase
- Animals
- B-Lymphocytes
(physiology)
- Disease Susceptibility
- Encephalomyelitis
(etiology)
- Enteritis
(etiology)
- Fas Ligand Protein
- Immunization, Passive
- Membrane Glycoproteins
(genetics)
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Protein-Tyrosine Kinases
(physiology)
- RNA, Messenger
(analysis)
- Viral Load
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