Protective immunity of SpaA-antigen producing Lactococcus lactis against Erysipelothrix rhusiopathiae infection.

Abstract

AIMS:

To develop an economical, safe and simple vaccination system against swine erysipelas using SpaA-antigen producing Lactococcus lactis.

METHODS AND RESULTS:

The spaA gene of Erysipelothrix rhusiopathiae was inserted into a shuttle plasmid pSECE1 to construct pSECE1.3. The SpaA produced in L. lactis maintained a stable antigenicity without degrading in growth. After mice were inoculated intranasally and orally with pSECE1.3-carrying L. lactis cells, IgG and IgA specific to SpaA were detected, and all the mice survived a challenge with 100 LD(50) of E. rhusiopathiae Tama-96 in the inner thigh.

CONCLUSIONS:

SpaA-producing L. lactis appears useful as an effective subunit vaccine against swine erysipelas.

SIGNIFICANCE AND IMPACT OF THE STUDY:

In this vaccination system, purification of the antigen and injection are unnecessary, leading to a reduced production cost, reduced labour and less stress to the animals. This vaccination system of the lactic acid bacteria should be a safe and suitable vehicle for a polyvalent vaccine.

• Authors: H I Cheun, K Kawamoto, M Hiramatsu, H Tamaoki, T Shirahata, S Igimi, S-I Makino
• Journal: Journal of applied microbiology (J Appl Microbiol) Vol. 96 Issue 6 Pg. 1347-53 ( 2004) ISSN: 1364-5072 [Print] England
• PMID: 15139928 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Bacterial
• Antigens, Bacterial
• Bacterial Proteins
• Bacterial Vaccines
• Immunoglobulin A
• Immunoglobulin G
• SpaA protein, Erysipelothrix rhusiopathiae
• Vaccines, DNA

Topics

• Administration, Intranasal
• Animals
• Antibodies, Bacterial (blood)
• Antigens, Bacterial (immunology)
• Bacterial Proteins (immunology)
• Bacterial Vaccines (immunology)
• Female
• Immunoglobulin A (blood)
• Immunoglobulin G (blood)
• Lactococcus lactis (immunology)
• Mice
• Plasmids
• Swine
• Swine Erysipelas (immunology, prevention & control)
• Vaccination (methods)
• Vaccines, DNA (immunology)