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CD8+ cell depletion of donor lymphocyte infusions using cd8 monoclonal antibody-coated high-density microparticles (CD8-HDM) after allogeneic hematopoietic stem cell transplantation: a pilot study.

Abstract
A CD8 murine monoclonal antibody-coated high-density microparticle (HDM) has been developed, which allows for the rapid depletion of CD8+ T cells from apheresis products by gravity sedimentation. We conducted a study to determine the efficacy and safety of CD8 depletion of donor lymphocyte infusions (DLI) to treat relapse after stem cell transplantation using the Eligix CD8-HDM Cell Separation System. Patients were targeted to receive 3 x 10(7) CD4+ T cells/kg. Nine patients were enrolled, three with CML, three myeloma, two CLL, and one NHL. A median of 1 x 10(10) mononuclear cells were obtained by apheresis and processed. The median depletion of CD8+ cells was 99.3% (97.8->99.5%). CD8 depletion was highly specific, with a median recovery of CD4+ cells of 75%. A median of 2.9 x 10(7) CD4+ cells/kg was infused. No infusional toxicity was noted. All CML patients achieved a complete molecular remission. A CLL patient demonstrated a complete response. One patient developed GVHD (grade II acute GVHD and subsequently chronic GVHD). The CD8-HDM Cell Separation System appears to be highly selective and effective in depleting CD8+ T cells from DLI apheresis products, and CD8-depleted DLI is capable of mediating a graft-versus-leukemia effect while minimizing GVHD.
AuthorsE P Alyea, C Canning, D Neuberg, H Daley, H Houde, S Giralt, R Champlin, K Atkinson, R J Soiffer
JournalBone marrow transplantation (Bone Marrow Transplant) Vol. 34 Issue 2 Pg. 123-8 (Jul 2004) ISSN: 0268-3369 [Print] England
PMID15133487 (Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Topics
  • Adult
  • Blood Component Removal (methods)
  • CD8-Positive T-Lymphocytes
  • Female
  • Hematologic Neoplasms (therapy)
  • Hematopoietic Stem Cell Transplantation (methods)
  • Humans
  • Immunomagnetic Separation
  • Lymphocyte Depletion (methods)
  • Lymphocyte Transfusion (methods)
  • Male
  • Middle Aged
  • Pilot Projects
  • Recurrence
  • Salvage Therapy (methods)
  • Transplantation, Homologous
  • Treatment Outcome

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