Abstract |
A CD8 murine monoclonal antibody-coated high-density microparticle (HDM) has been developed, which allows for the rapid depletion of CD8+ T cells from apheresis products by gravity sedimentation. We conducted a study to determine the efficacy and safety of CD8 depletion of donor lymphocyte infusions (DLI) to treat relapse after stem cell transplantation using the Eligix CD8-HDM Cell Separation System. Patients were targeted to receive 3 x 10(7) CD4+ T cells/kg. Nine patients were enrolled, three with CML, three myeloma, two CLL, and one NHL. A median of 1 x 10(10) mononuclear cells were obtained by apheresis and processed. The median depletion of CD8+ cells was 99.3% (97.8->99.5%). CD8 depletion was highly specific, with a median recovery of CD4+ cells of 75%. A median of 2.9 x 10(7) CD4+ cells/kg was infused. No infusional toxicity was noted. All CML patients achieved a complete molecular remission. A CLL patient demonstrated a complete response. One patient developed GVHD (grade II acute GVHD and subsequently chronic GVHD). The CD8-HDM Cell Separation System appears to be highly selective and effective in depleting CD8+ T cells from DLI apheresis products, and CD8-depleted DLI is capable of mediating a graft-versus- leukemia effect while minimizing GVHD.
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Authors | E P Alyea, C Canning, D Neuberg, H Daley, H Houde, S Giralt, R Champlin, K Atkinson, R J Soiffer |
Journal | Bone marrow transplantation
(Bone Marrow Transplant)
Vol. 34
Issue 2
Pg. 123-8
(Jul 2004)
ISSN: 0268-3369 [Print] England |
PMID | 15133487
(Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Topics |
- Adult
- Blood Component Removal
(methods)
- CD8-Positive T-Lymphocytes
- Female
- Hematologic Neoplasms
(therapy)
- Hematopoietic Stem Cell Transplantation
(methods)
- Humans
- Immunomagnetic Separation
- Lymphocyte Depletion
(methods)
- Lymphocyte Transfusion
(methods)
- Male
- Middle Aged
- Pilot Projects
- Recurrence
- Salvage Therapy
(methods)
- Transplantation, Homologous
- Treatment Outcome
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